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Autor
Almaas, Runar 1 Andersen, Elisabeth 1 Baumgarten, Saphira 1 Bezrouk, Ales 1 Bogen, Inger Lise 1 Boger, Ida C 1 Bonanini, Flavio 1 Bravo-Pérez, Carlos 1 Chollet, Maria Eugenia 1 Cizkova, Dana 1 Corral, Javier 1 Dejneka, Alexandr 1 Harrison, Sean P 1 Kempf, Henning 1 Kurek, Dorota 1 Lunov, Oleg 1 Mathapati, Santosh 1 Melum, Espen 1 Mokry, Jaroslav 1 Mowinckel, Marie-Christine 1
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Pracoviště
Department Leibniz Research Laborator... 1 Department of Chemistry University of... 1 Department of Engineering Faculty of ... 1 Department of Forensic Sciences Oslo ... 1 Department of Genetics Yale Stem Cell... 1 Department of Haematology Oslo Univer... 1 Department of Histology and Embryolog... 1 Department of Immunology University o... 1 Department of Medical Biophysics Facu... 1 Department of Medicine Faculty of Med... 1 Department of Molecular Medicine Inst... 1 Department of Optical and Biophysical... 1 Department of Pediatric Research Oslo... 1 European Reference Network RARE LIVER... 1 Hybrid Technology Hub Centre of Excel... 1 Institute of Clinical Medicine Facult... 1 Mimetas Leiden The Netherlands 1 Norwegian PSC Research Center Departm... 1 Research Institute of Internal Medici... 1 Section for Gastroenterology Departme... 1
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Autor
Almaas, Runar 1 Andersen, Elisabeth 1 Baumgarten, Saphira 1 Bezrouk, Ales 1 Bogen, Inger Lise 1 Boger, Ida C 1 Bonanini, Flavio 1 Bravo-Pérez, Carlos 1 Chollet, Maria Eugenia 1 Cizkova, Dana 1 Corral, Javier 1 Dejneka, Alexandr 1 Harrison, Sean P 1 Kempf, Henning 1 Kurek, Dorota 1 Lunov, Oleg 1 Mathapati, Santosh 1 Melum, Espen 1 Mokry, Jaroslav 1 Mowinckel, Marie-Christine 1
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Pracoviště
Department Leibniz Research Laborator... 1 Department of Chemistry University of... 1 Department of Engineering Faculty of ... 1 Department of Forensic Sciences Oslo ... 1 Department of Genetics Yale Stem Cell... 1 Department of Haematology Oslo Univer... 1 Department of Histology and Embryolog... 1 Department of Immunology University o... 1 Department of Medical Biophysics Facu... 1 Department of Medicine Faculty of Med... 1 Department of Molecular Medicine Inst... 1 Department of Optical and Biophysical... 1 Department of Pediatric Research Oslo... 1 European Reference Network RARE LIVER... 1 Hybrid Technology Hub Centre of Excel... 1 Institute of Clinical Medicine Facult... 1 Mimetas Leiden The Netherlands 1 Norwegian PSC Research Center Departm... 1 Research Institute of Internal Medici... 1 Section for Gastroenterology Departme... 1
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Free Medical Journals od 1996
Nature Open Access od 1996-03-01
PubMed Central od 2008
Europe PubMed Central od 2008
ProQuest Central od 2000-03-01
Health & Medicine (ProQuest) od 2000-03-01
PubMed
37653039
DOI
10.1038/s12276-023-01074-1
Knihovny.cz E-zdroje
The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes, rendering them less physiologically relevant compared to native tissue. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. Using single-cell RNA sequencing and immunofluorescence, we demonstrate a liver-like cellular repertoire, a higher order cellular complexity, presenting with vascular luminal structures, and a population of resident macrophages: Kupffer cells. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality. The organoids can be transplanted and maintained long term in mice producing human albumin. The organoids exhibit a complex cellular repertoire reflective of the organ and have de novo vascularization and liver-like function. These characteristics are a prerequisite for many applications from cellular therapy, tissue engineering, drug toxicity assessment, and disease modeling to basic developmental biology.
- MeSH
- hepatocyty MeSH
- játra * MeSH
- kultivované buňky MeSH
- lidé MeSH
- myši MeSH
- organoidy * MeSH
- tkáňové inženýrství MeSH
- zvířata MeSH
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- lidé MeSH
- myši MeSH
- zvířata MeSH
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- časopisecké články MeSH
- práce podpořená grantem MeSH
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