Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
- MeSH
- anaerobióza MeSH
- genetické vektory genetika MeSH
- Giardia lamblia cytologie genetika MeSH
- hydrolasy genetika MeSH
- ligandy MeSH
- mitochondrie metabolismus MeSH
- molekulární zobrazování metody MeSH
- organely metabolismus MeSH
- protozoální proteiny genetika MeSH
- rekombinantní fúzní proteiny genetika MeSH
- reportérové geny genetika MeSH
- Trichomonas vaginalis cytologie genetika MeSH
- viabilita buněk MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
