The research in vaccine development is growing very intensively. More than 95 clinical trials of potential HIV vaccines have been performed in last 25 years. Currently, only two HIV vaccine candidates are in Phase III trials and one in Phase II trial. Tuberculosis is another problematic disease of the 21st century. Calmette-Guérin vaccine from Mycobacterium bovis bacillus is the only one used for immunization against tuberculosis. Unfortunately, it is effective only in newborns but not in adults; thus, efforts are still put to prepare an effective vaccine for immunization and treatment of adults. Malaria killed more than 1 million people in 2006, most of them were children. We still do not have any vaccine effective in prevention of malaria; nevertheless, some vaccine candidates appeared and are now in Phase I or Phase II trial.
The worldwide growing interest to biomaterials over the last years results from their irreplaceable role in medical clinic. Hydroxyapatite is used in bone reconstruction because of its similar chemical structure compared to the inorganic composition of human bone and it is basic building component of many newly prepared biomaterials. In this study, we evaluated cytotoxic/antiproliferative activity of hydroxyapatite extract using murine fibroblast cell line NIH-3T3 and two in vitro different cytotoxic assays: growth inhibition assay and MTT assay. Hydroxyapatite extract after 72 h of incubation manifested the significant in vitro cytotoxic/antiproliferative effect only at the highest concentration tested (100 %). The antiproliferative effect of hydroxyapatite extract at the other concentrations tested (75 %, 50 %, 25 %, 10 %, 5 % and 1 %) was directly proportional to the concentration and the time of influence. The inhibition of cell proliferation was 86.8 - 0 %. The sensitivity of cell growth inhibition assay (direct counting of viable cells) to the extract influence was higher than that of MTT test.
- MeSH
- barvicí látky MeSH
- buňky NIH 3T3 MeSH
- hydroxyapatit toxicita MeSH
- kostní náhrady toxicita MeSH
- myši MeSH
- počet buněk MeSH
- proliferace buněk účinky léků MeSH
- testování materiálů MeSH
- tetrazoliové soli MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
