Conducting polyaniline can be prepared and modified using several procedures, all of which can significantly influence its applicability in different fields of biomedicine or biotechnology. The modifications of surface properties are crucial with respect to the possible applications of this polymer in tissue engineering or as biosensors. Innovative technique for preparing polyaniline films via in-situ polymerization in colloidal dispersion mode using four stabilizers (poly-N-vinylpyrrolidone; sodium dodecylsulfate; Tween 20 and Pluronic F108) was developed. The surface energy, conductivity, spectroscopic features, and cell compatibility of thin polyaniline films were determined using contact-angle measurement, the van der Pauw method, Fourier-transform infrared spectroscopy, and assay conducted on mouse fibroblasts, respectively. The stabilizers significantly influenced not only the surface and electrical properties of the films but also their cell compatibility. Sodium dodecylsulfate seems preferentially to combine both the high conductivity and good cell compatibility. Moreover, the films with sodium dodecylsulfate were non-irritant for skin, which was confirmed by their in-vitro exposure to the 3D-reconstructed human tissue model.

• MeSH
• aniliny škodlivé účinky   chemie   MeSH
• fibroblasty účinky léků   MeSH
• koloidy škodlivé účinky   chemie   MeSH
• membrány umělé * MeSH
• myši MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

The separation of Staphylococcus epidermidis and Rhodococcus erythropolis bacteria was achieved with the use of Micro-Thermal Focusing Field-Flow Fractionation. This is the first performance of separation exploiting the Ludwig-Soret effect (thermal diffusion) of living biological cells, combined with lift forces and resulting in the focusing mechanism of separation. The experiments were carried out under carefully chosen experimental conditions preventing the denaturation of the bacteria.

• MeSH
• difuze MeSH
• Rhodococcus izolace a purifikace   MeSH
• senzitivita a specificita MeSH
• Staphylococcus epidermidis izolace a purifikace   MeSH
• teoretické modely MeSH
• teplota MeSH
• termodynamika MeSH

• MeSH
• epidermis MeSH
• lidé MeSH
• povrchově aktivní látky MeSH
• salicylany farmakokinetika   MeSH
• techniky in vitro MeSH
• Check Tag
• lidé MeSH

• MeSH
• epidermis účinky léků   MeSH
• herbicidy chemie   škodlivé účinky   MeSH
• techniky in vitro MeSH