In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.
- MeSH
- antigeny bakteriální chemie genetika imunologie MeSH
- Borrelia burgdorferi chemie genetika imunologie MeSH
- mapování epitopu * MeSH
- multimerizace proteinu * MeSH
- myši inbrední BALB C MeSH
- myší monoklonální protilátky chemie imunologie MeSH
- myši MeSH
- proteiny vnější bakteriální membrány chemie genetika imunologie MeSH
- sbalování proteinů MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii).
- MeSH
- antigeny bakteriální genetika MeSH
- Borrelia burgdorferi komplex klasifikace MeSH
- DNA bakterií genetika MeSH
- flagelin genetika MeSH
- fylogeneze MeSH
- klíště mikrobiologie MeSH
- lymeská nemoc MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- proteiny vnější bakteriální membrány genetika MeSH
- sérotypizace * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
