Currently, the territorial Radiation Monitoring Network (RMN) of the Czech Republic consists of seven laboratories equipped with gamma spectrometry High Purity Germanium (HPGe) detectors. From 2007 to 2018, five emergency exercises were carried out to test the sample throughput of these facilities and their staff. The main objective was to identify weaknesses and problem areas in the whole process from the moment of obtaining the samples to logging the results into the central RMN database. The long-term aim of these exercises is to optimize emergency response procedures. The most important factor limiting laboratory capacity is the lack of qualified personnel. The exercises showed that in the current state, these laboratories would be able to operate in 12-hour shifts for 14 days and analyze 1700 samples per day. Emergency exercises have highlighted the fact that this type of exercise should be repeated periodically in order to monitor the performance and analytical capabilities of RMN.
- MeSH
- civilní obrana normy MeSH
- germanium MeSH
- laboratoře normy MeSH
- lidé MeSH
- monitorování radiace normy MeSH
- náhlé příhody * MeSH
- plánování postupu v případě katastrof organizace a řízení MeSH
- spektrometrie gama přístrojové vybavení metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Equine piroplasmosis caused by Babesia caballi and Theileria equi is widespread in Asia. The presence of these haemozoans in Mongolia was previously confirmed in domestic as well as in reintroduced Przewalski horses in which they cause significant pathology. The data on occurrence of piroplasms from Bactrian camels in Asia is lacking. A total of 192 horses, 70 Bactrian camels, and additional 16 shepherd dogs from the Hentiy province were included in our study. No clinical signs typical for piroplasmid infection were observed during the field survey. Microscopic examination revealed the presence of T. equi in blood smears from 67% of examined horses, with camels and dogs being negative. A two step PCR approach was used to detect piroplasms in peripheral blood. In the first "catch all" PCR reaction, amplification of the 496 bp-long fragment of the SSU rRNA gene enabled the detection of Babesia and Theileria spp. Second round multiplex PCR reaction used for species discrimination allowed the amplification of T. equi- and B. caballi-specific 340 bp and 650 bp-long regions of the SSU rRNA, respectively. This assay detected T. equi in 92.7% of horses, while the infections with B. caballi and dual infections were rare. In both PCR setups, camels and dogs were negative indicating that in the studied region, these hosts do not share piroplasms with horses.
- MeSH
- babezióza epidemiologie parazitologie veterinární MeSH
- koně MeSH
- nemoci koní epidemiologie parazitologie MeSH
- nemoci psů epidemiologie parazitologie MeSH
- psi MeSH
- velbloudi MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- psi MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Mongolsko MeSH
Two experimental trials were performed to elucidate the role of rodents in the life cycle of Hepatozoon species using snakes as intermediate hosts. In one trial, two ball pythons, Python regius Shaw, 1802 were force fed livers of laboratory mice previously inoculated with sporocysts of Hepatozoon ayorgbor Sloboda, Kamler, Bulantová, Votýpka et Modrý, 2007. Transmission was successful in these experimentally infected snakes as evidenced by the appearance of intraerythrocytic gamonts, which persisted until the end of trial, 12 months after inoculation. Developmental stages of haemogregarines were not observed in histological sections from mice. In another experimental trial, a presence of haemogregarine DNA in mice inoculated with H. ayorgbor was demonstrated by PCR in the liver, lungs and spleen.
