We announce the completion of the genome sequence of a phenol derivative-degrading bacterium, Rhodococcus erythropolis strain CCM2595. This bacterium is interesting in the context of bioremediation for its capability to degrade phenol, catechol, resorcinol, hydroxybenzoate, hydroquinone, p-chlorophenol, p-nitrophenol, pyrimidines, and sterols.
- Publikační typ
- časopisecké články MeSH
Achromobacter xylosoxidans strain A8 was isolated from soil contaminated with polychlorinated biphenyls. It can use 2-chlorobenzoate and 2,5-dichlorobenzoate as sole sources of carbon and energy. This property makes it a good starting microorganism for further development toward a bioremediation tool. The genome of A. xylosoxidans consists of a 7-Mb chromosome and two large plasmids (98 kb and 248 kb). Besides genes for the utilization of xenobiotic organic substrates, it contains genes associated with pathogenesis, toxin production, and resistance. Here, we report the complete genome sequence.
- MeSH
- Achromobacter denitrificans genetika izolace a purifikace metabolismus MeSH
- bakteriální chromozomy MeSH
- chlorbenzoáty metabolismus MeSH
- DNA bakterií chemie genetika MeSH
- energetický metabolismus MeSH
- genom bakteriální MeSH
- molekulární sekvence - údaje MeSH
- plazmidy MeSH
- půdní mikrobiologie MeSH
- sekvenční analýza DNA MeSH
- uhlík metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Epithelial-mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples.
- MeSH
- buňky NIH 3T3 MeSH
- čipová analýza proteinů MeSH
- fenotyp MeSH
- fibroblasty metabolismus MeSH
- imunohistochemie MeSH
- insulinu podobný růstový faktor II biosyntéza MeSH
- keratinocyty cytologie metabolismus MeSH
- kostní morfogenetický protein 4 biosyntéza MeSH
- lidé MeSH
- myši MeSH
- nádory hlavy a krku metabolismus patologie MeSH
- rekombinantní proteiny biosyntéza MeSH
- spinocelulární karcinom metabolismus patologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
