Changes in the abundance of bacteria and fungi and in the composition of bacterial communities during primary succession were investigated in a brown coal mine deposit area near Sokolov, the Czech Republic, using phospholipid fatty acids analysis, microarray and 16S rRNA gene sequencing. The study considered a chronosequence of sites undergoing spontaneous succession: 6-, 12-, 21- and 45-year-old and a 21-year-old site revegetated with Alnus glutinosa. During succession, organic carbon and the total nitrogen content increased while the pH and the C/N ratio decreased. Microbial biomass and bacterial diversity increased until 21 years and decreased later; bacteria dominated over fungi in the initial and late phases of succession. Bacterial community composition of the 6-year-old site with no vegetation cover largely differed from the older sites, especially by a higher content of Gammaproteobacteria, Cyanobacteria and some Alphaproteobacteria. Bacteria belonging to the genera Acidithiobacillus, Thiobacillus and related taxa, the CO(2) and N(2) fixers, dominated the community at this site. In the later phases, bacterial community development seemed to reflect more the changes in soil nutrient content and pH than vegetation with a decrease of Actinobacteria and an increase of Acidobacteria. The site revegetated with A. glutinosa resembled the 45-year-old primary succession site and exhibited an even lower pH and C/N ratio, indicating that recultivation is able to accelerate soil development.

• MeSH
• Acidobacteria MeSH
• Actinobacteria genetika   růst a vývoj   MeSH
• Alphaproteobacteria genetika   růst a vývoj   MeSH
• Bacteria klasifikace   genetika   růst a vývoj   MeSH
• biodiverzita MeSH
• biomasa MeSH
• DNA bakterií analýza   MeSH
• dusík analýza   metabolismus   MeSH
• fylogeneze MeSH
• geny rRNA MeSH
• houby genetika   růst a vývoj   MeSH
• molekulární sekvence - údaje MeSH
• monitorování životního prostředí MeSH
• půda chemie   MeSH
• půdní mikrobiologie MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvence nukleotidů MeSH
• sinice genetika   růst a vývoj   MeSH
• těžba uhlí MeSH
• uhlí MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH