- MeSH
- analýza polymorfismu délky amplifikovaných restrikčních fragmentů metody MeSH
- DNA bakterií genetika chemie MeSH
- DNA fingerprinting metody MeSH
- ekosystém MeSH
- izotopové značení MeSH
- membránové lipidy analýza MeSH
- mikrobiologie životního prostředí MeSH
- pulzní gelová elektroforéza metody MeSH
- sekvenční analýza DNA metody MeSH
Liquid cultures with cellulose and solid state fermentation cultures on wheat straw of the white-rot fungi Pleurotus ostreatus and Trametes versicolor and the brown-rot fungus Piptoporus betulinus were assayed for the free and solid fraction-bound activity of lignocellulose-degrading enzymes. The majority of the ligninolytic enzymes laccase and Mn peroxidase was detected in the free fraction of P. ostreatus and T. versicolor. The endocleaving enzymes endo-1,4-beta-glucanase, endo-1,4-beta-mannanase and endo-1,4-beta-xylanase were detected almost exclusively in the free fraction, while significant amounts of 1,4-beta-glucosidase, cellobiohydrolase, 1,4-beta-xylosidase and 1,4-beta-mannosidase were present in the bound fraction depending on the mode of cultivation and the species. The bound enzymes accounted for 66% of the total activity in P. ostreatus straw cultures, 35% in T. versicolor and only 8% in P. betulinus. The enzymes also showed significant differences in freeze-drying stability. Hydrolases in general showed high stability, whereas laccase and Mn peroxidase of P. ostreatus were the least stable.
- MeSH
- celulasy analýza MeSH
- celulosa metabolismus MeSH
- enzymy analýza MeSH
- financování organizované MeSH
- fungální proteiny analýza MeSH
- glykosidhydrolasy analýza MeSH
- kultivační média chemie MeSH
- lakasa analýza MeSH
- lignin metabolismus MeSH
- lyofilizace MeSH
- peroxidasy analýza MeSH
- Pleurotus enzymologie MeSH
- Polyporales enzymologie MeSH
- stabilita enzymů MeSH
