Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Promyelocytic leukemia nuclear bodies (PML NBs), the structural domains of the eukaryotic cell nucleus, play a role in cancer and apoptosis, and their involvement in antiviral mechanisms mediated by interferons (IFNs) is proposed. IFNs dramatically increase the transcription of the PML gene. In this study, we have shown that the response of 2 structural PML NB components, PML and Sp100, to interferon-alpha (IFNalpha) was suppressed in cells simultaneously treated with histone deacetylase (HDAC) inhibitors (trichostatin A, sodium butyrate, MS-275, SAHA, and valproic acid). Trichostatin A (TSA) blocked the increase of PML NB number and suppressed up-regulation of PML mRNA and protein levels in several human cell lines and in normal diploid skin fibroblasts. Moreover, IFNalpha induction of IRF-1 was also inhibited by TSA, although incompletely. Promyelocytic leukemia nuclear bodies (PML NBs), the structural domains of the eukaryotic cell nucleus, play a role in cancer and apoptosis, and their involvement in antiviral mechanisms mediated by interferons (IFNs) is proposed. IFNs dramatically increase the transcription of the PML gene. In this study, we have shown that the response of 2 structural PML NB components, PML and Sp100, to interferon-alpha (IFNalpha) was suppressed in cells simultaneously treated with histone deacetylase (HDAC) inhibitors (trichostatin A, sodium butyrate, MS-275, SAHA, and valproic acid). Trichostatin A (TSA) blocked the increase of PML NB number and suppressed up-regulation of PML mRNA and protein levels in several human cell lines and in normal diploid skin fibroblasts. Moreover, IFNalpha induction of IRF-1 was also inhibited by TSA, although incompletely.
- MeSH
- aktivace transkripce účinky léků MeSH
- financování organizované MeSH
- histondeacetylasy MeSH
- inhibitory enzymů farmakologie MeSH
- inhibitory histondeacetylas MeSH
- interferon alfa farmakologie MeSH
- jaderné proteiny genetika MeSH
- kultivované buňky MeSH
- lidé MeSH
- nádorové buňky kultivované MeSH
- nádorové proteiny genetika MeSH
- nádorové supresorové proteiny genetika MeSH
- RNA nádorová analýza MeSH
- transkripční faktor STAT2 metabolismus MeSH
- transkripční faktory genetika MeSH
- upregulace účinky léků MeSH
- Check Tag
- lidé MeSH
