Neurologie pro praxi, ISSN 1213-1814 ; Ročník 2022, číslo 23, Supplementum CH
14 stran : ilustrace ; 30 cm
Brožura shrnuje klinické protokoly a doporučení pro diagnostiku a monitorování roztroušené sklerózy pomocí magnetické rezonance. Určeno odborné veřejnosti.
- MeSH
- klinické protokoly MeSH
- kontrastní látky MeSH
- magnetická rezonanční tomografie metody MeSH
- neurofyziologický monitoring MeSH
- roztroušená skleróza diagnostické zobrazování MeSH
- směrnice pro lékařskou praxi jako téma MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- neurologie
- radiologie, nukleární medicína a zobrazovací metody
- NLK Publikační typ
- brožury
Mouse activating Nkrp1 proteins are commonly described as type II transmembrane receptors with disulfide-linked homodimeric structure. Their function and the manner in which Nkrp1 proteins of mouse strain (C57BL/6) oligomerize are still poorly understood. To assess the oligomerization state of Nkrp1 proteins, mouse activating EGFP-Nkrp1s were expressed in mammalian lymphoid cells and their oligomerization evaluated by Förster resonance energy transfer (FRET). Alternatively, Nkrp1s oligomers were detected by Western blotting to specify the ratio between monomeric and dimeric forms. We also performed structural characterization of recombinant ectodomains of activating Nkrp1 receptors. Nkrp1 isoforms c1, c2 and f were expressed prevalently as homodimers, whereas the Nkrp1a displays larger proportion of monomers on the cell surface. Cysteine-to-serine mutants revealed the importance of all stalk cysteines for protein dimerization in living cells with a major influence of cysteine at position 74 in two Nkrp1 protein isoforms. Our results represent a new insight into the oligomerization of Nkrp1 receptors on lymphoid cells, which will help to determine their function.
- MeSH
- antigeny Ly analýza MeSH
- Cercopithecus aethiops MeSH
- COS buňky MeSH
- Jurkat buňky MeSH
- lektinové receptory NK-buněk - podrodina B analýza MeSH
- lidé MeSH
- multimerizace proteinu MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- receptory imunologické analýza MeSH
- refolding proteinů MeSH
- rezonanční přenos fluorescenční energie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
We have recently identified a new class of high affinity ligands for CD69 leukocyte membrane receptor, carboxylated calixarenes. Of the three compounds investigated here, thiacalix[4]arene had the highest affinity for CD69 in direct binding assays, and proved to be the most specific inhibitor of CD69 identified so far in receptor precipitation and cellular activation experiments. Carboxylated calixarenes also proved effective at protection of CD69(high) lymphocytes from apoptosis triggered by a multivalent ligand or antibody. Thus, carboxylated calixarenes set a new paradigm for noncarbohydrate ligands for CD69 making them attractive for protection of killer cells in combined animal tumor therapies.
- MeSH
- apoptóza MeSH
- CD antigeny metabolismus MeSH
- diferenciační antigeny T-lymfocytů metabolismus MeSH
- kalixareny chemie metabolismus MeSH
- krysa rodu rattus MeSH
- kyseliny karboxylové chemie MeSH
- lektiny typu C metabolismus MeSH
- lidé MeSH
- ligandy MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- publikace stažené z tisku MeSH
