Proteins MiaA and MiaB catalyze sequential isopentenylation and methylthiolation, respectively, of adenosine residue in 37th position of tRNAXXA. The mia mutations were recently shown by us to affect secondary metabolism and morphology of Streptomyces. However, it remained unknown as to whether both or one of the aforementioned modifications is critical for colony development and antibiotic production. Here, we addressed this issue through analysis of Streptomyces albus J1074 strains carrying double miaAmiaB knockout or extra copy of miaB gene. The double mutant differed from wild-type and miaA-minus strains in severity of morphological defects, growth dynamics, and secondary metabolism. Introduction of extra copy of miaB gene into miaA mutant restored aerial mycelium formation to the latter on certain solid media. Hence, miaB gene might be involved in tRNA thiomethylation in the absence of miaA; either MiaA- or MiaB-mediated modification appears to be enough to support normal metabolic and morphological processes in Streptomyces.

• MeSH
• alkyltransferasy a aryltransferasy genetika   MeSH
• antibakteriální látky biosyntéza   MeSH
• bakteriální geny genetika   MeSH
• bakteriální proteiny genetika   MeSH
• fenotyp * MeSH
• genetická transkripce MeSH
• genetické testování metody   MeSH
• mutace MeSH
• peroxid vodíku farmakologie   MeSH
• posttranskripční úpravy RNA * MeSH
• RNA transferová metabolismus   MeSH
• sekundární metabolismus účinky léků   genetika   MeSH
• Streptomyces účinky léků   genetika   růst a vývoj   metabolismus   MeSH
• sulfurtransferasy genetika   MeSH
• Publikační typ
• časopisecké články MeSH

The cell wall of the model actinomycete Streptomyces coelicolor M145 has recently been shown to contain the novel glycopolymer teichulosonic acid. The major building block of this polymer is 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (Kdn), suggesting initial clues about the genetic control of biosynthesis of this cell wall component. Here, through genome mining and gene knockouts, we demonstrate that the sco4879-sco4882 genomic region of S. coelicolor M145 is necessary for biosynthesis of teichulosonic acid. Specifically, mutants carrying individual knockouts of sco4879, sco4880 and sco4881 genes do not produce Kdn-containing glycopolymer and instead accumulate the minor cell wall component poly(diglycosyl 1-phosphate). Our studies provide evidence that this region is at least partly responsible for biosynthesis of Kdn, whereas flanking genes might control the other steps of teichulosonic acid formation.

• MeSH
• bakteriální polysacharidy biosyntéza   MeSH
• buněčná stěna genetika   metabolismus   MeSH
• data mining MeSH
• DNA bakterií genetika   MeSH
• inzerční mutageneze MeSH
• klonování DNA MeSH
• kyseliny cukerné metabolismus   MeSH
• magnetická rezonanční spektroskopie MeSH
• Streptomyces coelicolor genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH