The inter- and intraspecies variability of lactate dehydrogenase (ldh) gene was determined among the predominant ruminal lactate utilizing bacteria. Nearly complete nucleotide sequences of ldh gene, encoding NAD-dependent lactate dehydrogenase of three Megasphaera elsdenii and six Selenomonas ruminantium strains, were obtained and compared. Phylogenetic analyses revealed a limited variability between the ldh sequences studied. The majority of differences observed were silent mutations at the 3rd position of codons. Surprisingly, the intraspecies diversity of the ldh gene among S. ruminantium isolates was higher than the interspecies level between S. ruminantium and M. elsdenii, which strongly suggests the possibility of acquisition of this gene by horizontal gene transfer.
- MeSH
- acidóza laktátová metabolismus MeSH
- bachor mikrobiologie MeSH
- bakteriální proteiny genetika MeSH
- bodová mutace MeSH
- DNA bakterií genetika chemie MeSH
- fylogeneze MeSH
- genetická variace MeSH
- L-laktátdehydrogenasa genetika MeSH
- Megasphaera MeSH
- molekulární sekvence - údaje MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie MeSH
- Selenomonas MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
