Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

• MeSH
• antigeny bakteriální chemie   imunologie   MeSH
• CHO buňky MeSH
• Cricetulus MeSH
• druhová specificita MeSH
• glykomika MeSH
• imunitní systém MeSH
• lektiny MeSH
• lidé MeSH
• mikročipová analýza metody   MeSH
• oligosacharidy MeSH
• polysacharidy chemie   klasifikace   imunologie   MeSH
• rekombinantní proteiny MeSH
• transportní proteiny chemie   MeSH
• vazba proteinů MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH