The endothelin axis (endothelins and their receptors) is strongly involved in physiological and pathological processes. ET-1 plays a crucial role in particular in tumor diseases. Endothelin-1 receptors (ET(A) and ET(B)) are deregulated and overexpressed in several tumors such as melanoma and glioma. We studied the binding of 24 monoclonal antibodies directed against human ET(B) receptors (hET(B)) to different melanoma cell lines. Few of these mAbs bound to all the melanoma cell lines. One of them, rendomab B49, bound to ET(B) receptors expressed at the surface of human glioma stem cells. More recently, we produced new antibodies directed against human ET(A) receptor (hET(A)). Several antibodies have been isolated and have been screened on different tumoral cells lines. As for the mAbs directed against the hET(B) receptor only some of new antibodies directed against ET(A) receptor are capable to bind the human tumoral cell lines. Rendomab A63 directed against hET(A) is one of them. We report the specificity and binding properties of these mAbs and consider their potential use in diagnosis by an in vivo imaging approach.
- MeSH
- antagonisté endotelinového receptoru A aplikace a dávkování metabolismus MeSH
- CHO buňky MeSH
- Cricetulus MeSH
- endotelin-1 genetika metabolismus MeSH
- křečci praví MeSH
- lidé MeSH
- monoklonální protilátky aplikace a dávkování metabolismus MeSH
- myši nahé MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- receptor endotelinu A genetika metabolismus MeSH
- receptor endotelinu B genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- vazba proteinů fyziologie MeSH
- xenogenní modely - testy antitumorózní aktivity metody MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH