Phytophthora plurivora and other Phytophthora species are known to be serious pathogens of forest trees. Little is known, however, about the presence of P. plurivora in Polish oak forests and their role in oak decline. The aims of this study were to identify P. plurivora in healthy and declining Quercus robur stands in southern Poland and to demonstrate the relationship between different site factors and the occurrence of P. plurivora. In addition, the virulence of P. plurivora and other Phytophthora species was evaluated through inoculations using 2-year-old oak seedlings. Rhizosphere soil was investigated from 39 oak stands representing different healthy tree statuses. The morphology and DNA sequences of the internal transcribed spacer regions (ITS) of the ribosomal DNA and the mitochondrial cox1 gene were used for identifications. P. plurivora, an oak fine root pathogen, was isolated from rhizosphere soil samples in 6 out of 39 stands. Additionally, Phytophthora cambivora, Phytophthora polonica and Phytophthora rosacearum-like were also obtained from several stands. The results showed a significant association between the presence of P. plurivora and the health status of oak trees. Similar relationships were also observed for all identified Phytophthora species. In addition, there was evidence for a connection between the presence of all identified Phytophthora species and some site conditions. Phytophthora spp. occurred more frequently in declining stands and in silt loam and sandy loam soils with pH ≥ 3.66. P. plurivora and P. cambivora were the only species capable of killing whole plants, producing extensive necrosis on seedling stems.

• MeSH
• dub (rod) parazitologie   MeSH
• fylogeneze MeSH
• kořeny rostlin parazitologie   MeSH
• lesy MeSH
• molekulární sekvence - údaje MeSH
• nemoci rostlin parazitologie   MeSH
• Phytophthora klasifikace   genetika   izolace a purifikace   MeSH
• stromy parazitologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Polsko MeSH

The ophiostomatoid fungi associated with cerambycid beetles Tetropium spp. (their symbiotic vectors) colonizing Norway spruce in Poland (six species collected) were isolated. The virulence of representative isolates was evaluated through inoculations using 2-year-old Norway spruce seedlings. A total of 1325 isolates (Ophiostoma piceae, O. tetropii, O. minus, Grosmannia piceiperda, G. cucullata, and five other less frequent taxa) were obtained. Tetropium castaneum and T. fuscum were vectors of similar spectra of ophiostomatoid fungi although some differences in fungal frequency between these Tetropium spp. were found. Among the fungal associates of the Tetropium spp. collected only G. piceiperda was pathogenic, which suggests that it can play a role in the death of spruce trees following attack by Tetropium spp.

• MeSH
• biodiverzita MeSH
• brouci mikrobiologie   MeSH
• financování organizované MeSH
• fylogeneze MeSH
• hmyz - vektory mikrobiologie   MeSH
• molekulární sekvence - údaje MeSH
• nemoci rostlin mikrobiologie   parazitologie   MeSH
• Ophiostomatales MeSH
• smrk mikrobiologie   parazitologie   MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Geografické názvy
• Polsko MeSH