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Autor
Bernecky, Carrie 1 Buccheri, Valeria 1 Horvat, Filip 1 Jenickova, Irena 1 Joseph, Diego F 1 Kubicek, Karel 1 Malik, Radek 1 Much, Christian 1 Novacek, Jiri 1 O'Carroll, Dónal 1 Pasulka, Josef 1 Pinkas, Matyas 1 Prochazka, Jan 1 Prochazkova, Michaela 1 Sebesta, Marek 1 Sedlacek, Radislav 1 Stefl, Richard 1 Svoboda, Petr 1 Taborska, Eliska 1 Zanova, Martina 1
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Pracoviště
Bioinformatics Group Department of Bi... 1 CEITEC Central European Institute of ... 1 Centre for Regenerative Medicine Inst... 1 Czech Centre for Phenogenomics and La... 1 European Molecular Biology Laboratory... 1 Institute of Molecular Genetics of th... 1 Institute of Science and Technology A... 1 National Centre for Biomolecular Rese... 1 Wellcome Centre for Cell Biology Scho... 1
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Autor
Bernecky, Carrie 1 Buccheri, Valeria 1 Horvat, Filip 1 Jenickova, Irena 1 Joseph, Diego F 1 Kubicek, Karel 1 Malik, Radek 1 Much, Christian 1 Novacek, Jiri 1 O'Carroll, Dónal 1 Pasulka, Josef 1 Pinkas, Matyas 1 Prochazka, Jan 1 Prochazkova, Michaela 1 Sebesta, Marek 1 Sedlacek, Radislav 1 Stefl, Richard 1 Svoboda, Petr 1 Taborska, Eliska 1 Zanova, Martina 1
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Pracoviště
Bioinformatics Group Department of Bi... 1 CEITEC Central European Institute of ... 1 Centre for Regenerative Medicine Inst... 1 Czech Centre for Phenogenomics and La... 1 European Molecular Biology Laboratory... 1 Institute of Molecular Genetics of th... 1 Institute of Science and Technology A... 1 National Centre for Biomolecular Rese... 1 Wellcome Centre for Cell Biology Scho... 1
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- Zapletal, David
- Taborska, Eliska
- Pasulka, Josef
- Malik, Radek
- Kubicek, Karel
- Zanova, Martina
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Much, Christian
Autor Much, Christian Centre for Regenerative Medicine, Institute for Regeneration and Repair, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK European Molecular Biology Laboratory (EMBL), Mouse Biology Unit, Via Ramarini 32, Monterotondo Scalo 00015, Italy
- Sebesta, Marek
- Buccheri, Valeria
- Horvat, Filip
Free Medical Journals od 1997 do Před 1 rokem
Free Medical Journals od 1997 do Před 1 rokem
Open Access Digital Library od 1997-12-01
Elsevier Open Archive Journals od 1997-12-01 do Před 1 rokem
PubMed
36332606
DOI
10.1016/j.molcel.2022.10.010
Knihovny.cz E-zdroje
MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucleases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is specifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer's DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the complete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicer•-miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleavage-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways.
- MeSH
- mikro RNA * genetika metabolismus MeSH
- myši MeSH
- ribonukleasa III * metabolismus MeSH
- RNA interference MeSH
- savci metabolismus MeSH
- transportní proteiny metabolismus MeSH
- zvířata MeSH
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- práce podpořená grantem MeSH
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