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7 záznamů v Medvik Filtry

Článek

External Quality Assurance Schemes (EQUASs) and Inter-laboratory Comparison Investigations (ICIs) for human biomonitoring of polycyclic aromatic hydrocarbon (PAH) biomarkers in urine as part of the quality assurance programme under HBM4EU

Nübler, Stefanie
Autor Nübler, Stefanie Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany
Esteban López, Marta
Autor Esteban López, Marta National Center for Environmental Health, Instituto de Salud Carlos III, Ctra. Majadahonda a Pozuelo Km2,2, 28220, Madrid, Spain
Castaño, Argelia
Autor Castaño, Argelia National Center for Environmental Health, Instituto de Salud Carlos III, Ctra. Majadahonda a Pozuelo Km2,2, 28220, Madrid, Spain
Mol, Hans G J
Autor Mol, Hans G J Wageningen Food Safety Research, Part of Wageningen University and Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands
Müller, Johannes
Autor Müller, Johannes Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany
Schäfer, Moritz
Autor Schäfer, Moritz Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany
Haji-Abbas-Zarrabi, Karin
Autor Haji-Abbas-Zarrabi, Karin Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany
Hajslova, Jana
Autor Hajslova, Jana University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic
Pulkrabová, Jana, 1980-
Autor Autorita University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic
Dvorakova, Darina
Autor Dvorakova, Darina University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic

International journal of hygiene and environmental health. 2023 ; 250 (-) : 114169. [pub] 20230424

Int J Hyg Environ Health
ISSN 1618-131X
Medvik
Zdroj

Polycyclic aromatic hydrocarbons (PAHs) were included as priority substances for human biomonitoring (HBM) in the European Human Biomonitoring Initiative (HBM4EU), which intended to harmonise and advance HBM across Europe. For this project, a specific Quality Assurance and Quality Control (QA/QC) programme applying Inter-laboratory Comparison Investigations (ICIs) and External Quality Assurance Schemes (EQUASs) was developed to ensure the comparability and accuracy of participating analytical laboratories. This paper presents the results of four ICI/EQUAS rounds for the determination of 13 PAH metabolites in urine, i.e. 1-naphthol, 2-naphthol, 1,2-dihydroxynaphthalene, 2-, 3- and 9-hydroxyfluorene, 1-, 2-, 3-, 4- and 9-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenzo(a)pyrene. However, 4 PAH metabolites could not be evaluated as the analytical capacity of participating laboratories was too low. Across all rounds and biomarkers, 86% of the participants achieved satisfactory results, although low limits of quantification were required to quantify the urinary metabolites at exposure levels of the general population. Using high-performance liquid or gas chromatography coupled with mass spectrometry (HPLC-MS; GC-MS) and isotope dilution for calibration as well as performing an enzymatic deconjugation step proved to be favourable for the accurate determination of PAHs in urine. Finally, the HBM4EU QA/QC programme identified an international network of laboratories providing comparable results in the analysis of urinary PAH biomarkers, although covering all parameters initially selected was still too challenging.