The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types.
- MeSH
- Argonaut proteiny genetika metabolismus MeSH
- Drosophila melanogaster genetika MeSH
- iniciační faktory genetika metabolismus MeSH
- malá interferující RNA genetika metabolismus MeSH
- ovarium fyziologie MeSH
- proteiny asociované s mikrotubuly genetika metabolismus MeSH
- proteiny Drosophily genetika metabolismus MeSH
- reprodukovatelnost výsledků MeSH
- RNA interference * MeSH
- stanovení celkové genové exprese metody MeSH
- transpozibilní elementy DNA * MeSH
- umlčování genů MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
