• Publikační typ
• abstrakt z konference MeSH

OBJECTIVE: To assess the effects of intravenous (IV) medetomidine-butorphanol and IV dexmedetomidine-butorphanol on intraocular pressure (IOP). STUDY DESIGN: Prospective, randomized, blinded clinical study. ANIMALS: Forty healthy dogs. Mean ± SD body mass 37.6 ± 6.6 kg and age 1.9 ± 1.3 years. METHODS: Dogs were allocated randomly to receive an IV combination of dexmedetomidine, 0.3 mg m(-2), combined with butorphanol, 6 mg m(-2), (group DEX) or medetomidine 0.3 mg m(-2), combined with butorphanol 6 mg m(-2), (group MED). IOP and pulse (PR) and respiratory (f(R) ) rates were measured prior to (baseline) and at 10 (T10), 20 (T20), 30 (T30) and 40 (T40) minutes after drug administration. Oxygen saturation of hemoglobin (SpO(2)) was monitored following sedation. Data were analyzed by anova followed by Dunnett's tests for multiple comparisons. Changes were considered significant when p < 0.05. RESULTS: Following drug administration, PR and f(R) were decreased significantly at all time points but did not differ significantly between groups. Baseline IOP in mmHg was 14 ± 2 for DEX and 13 ± 2 for MED. With both treatments, at T10, IOP increased significantly (p < 0.001), reaching 20 ± 3 and 17 ± 2 for DEX and MED respectively. This value for DEX was significantly higher than for MED. There were no significant differences in IOP values between groups at any other time points. At T30 and T40, IOP in both groups was below baseline (DEX, 12 ± 2 and 11 ± 2: MED 12 ± 2 and 11 ± 2) and this was statistically significant, for DEX. CONCLUSIONS AND CLINICAL RELEVANCE: At the documented doses, both sedative combinations induced a transient increase and subsequent decrease of IOP relative to baseline, which must be taken into consideration when planning sedation of animals in which marked changes in IOP would be undesirable.

• MeSH
• butorfanol aplikace a dávkování   farmakologie   MeSH
• časové faktory MeSH
• dexmedetomidin aplikace a dávkování   farmakologie   MeSH
• kombinovaná farmakoterapie MeSH
• medetomidin aplikace a dávkování   farmakologie   MeSH
• neopioidní analgetika aplikace a dávkování   farmakologie   MeSH
• nitrooční tlak účinky léků   MeSH
• opioidní analgetika aplikace a dávkování   farmakologie   MeSH
• psi fyziologie   MeSH
• zvířata MeSH
• Check Tag
• psi fyziologie   MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH

The present study determined markers of oxidative and antioxidative activity in dog females affected with mammary gland tumour compared to healthy ones. The effect of additional vitamin E supplementation on oxidative and antioxidative status was evaluated as well. The study included 29 female dogs divided into 4 groups (groups 1 and 2 included females with a mammary gland tumour; groups 3 and 4 included healthy female dogs). Additional vitamin supplement containing α-tocopherol was given to the females of groups 1 and 4. Dogs from groups 1 and 2 were anaesthetized before surgery (ovariohysterectomy and mastectomy); anaesthesia was used also in group 3, but without performeing surgery. The content of vitamin E (free α-tocopherol), marker of antioxidative status, was measured in blood serum by liquid chromatography. The concentration of thiobarbituric acid reactive substances, marker of oxidative status, in serum and concentrations of protein and non-protein thiol groups, markers of oxidative and antioxidative status, in blood serum and in red blood cells were measured colorimetrically. In females with a mammary gland tumour from group 2, concentration of thiobarbituric acid reactive substances was significantly higher than 14 days after surgery and compared to healthy ones as well. In females with a mammary gland tumour from group 2, concentration of protein thiol groups in serum was significantly lower and concentration of non-protein thiol groups in serum was significantly higher than in healthy controls. The values of protein thiols in erythrocytes in females with mammary gland tumour from group 1 were significantly higher before supplementation with vitamin E. The present study revealed that females with a mammary gland tumour were more burdened with oxidative stress compared to healthy dogs. The removal of the mammary gland tumour led to improvement of oxidative and antioxidative status. This is the first report focusing on the effect of additional α-tocopherol supplementation on reducing oxidative stress by increasing antioxidative activity in females affected with mammary gland tumour; however, we did not prove it.

• MeSH
• alfa-tokoferol * farmakologie   krev   terapeutické užití   MeSH
• antioxidancia MeSH
• biologické markery * krev   MeSH
• hysterektomie MeSH
• látky reagující s kyselinou thiobarbiturovou izolace a purifikace   MeSH
• mastektomie MeSH
• nádory mléčné žlázy u zvířat * farmakoterapie   chirurgie   MeSH
• ovarektomie MeSH
• oxidační stres účinky léků   MeSH
• psi MeSH
• sulfhydrylové sloučeniny klasifikace   krev   MeSH
• vitamin E MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH
• srovnávací studie MeSH