Bacteria have developed different intra- and inter-specific communication mechanisms that involve the production, release, and detection of signaling molecules, because these molecules serve as the autoinducers involved in "quorum sensing" systems. Other communication mechanisms employ volatile signaling molecules that regulate different bacterial processes. The Arthrobacter agilis strain UMCV2 is a plant growth promoting actinobacterium, which induces plant growth and inhibits phytopathogenic fungi by emitting the dimethylhexadecylamine (DMHDA). However, little is known about the effect of this volatile compound on A. agilis UMCV2 itself, as well as on other bacteria. By exposing A. agilis UMCV2 and bacteria of the genus Bacillus and Pseudomonas to different concentrations of DMHDA, this study showed the dose-dependent effects of DMHDA on A. agilis UMCV2 growth, cellular viability, swarming motility, and expression of marker genes of the flagellar apparatus of bacteria. DMHDA was found to also modulate swarming motility of Bacillus sp. ZAP018 and P. fluorescens UM270, but not that of P. aeruginosa PA01. These data indicate that DMHDA is involved in both intra- and inter-specific bacterial interaction.

• MeSH
• Arthrobacter účinky léků   růst a vývoj   MeSH
• Bacillus účinky léků   růst a vývoj   MeSH
• methylaminy farmakologie   MeSH
• mikrobiální interakce účinky léků   MeSH
• pohyb účinky léků   MeSH
• Pseudomonas účinky léků   růst a vývoj   MeSH
• quorum sensing účinky léků   MeSH
• těkavé organické sloučeniny farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH

Medicago truncatula represents a model plant species for understanding legume-bacteria interactions. M. truncatula roots form a specific root-nodule symbiosis with the nitrogen-fixing bacterium Sinorhizobium meliloti. Symbiotic nitrogen fixation generates high iron (Fe) demands for bacterial nitrogenase holoenzyme and plant leghemoglobin proteins. Leguminous plants acquire Fe via "Strategy I," which includes mechanisms such as rhizosphere acidification and enhanced ferric reductase activity. In the present work, we analyzed the effect of S. meliloti volatile organic compounds (VOCs) on the Fe-uptake mechanisms of M. truncatula seedlings under Fe-deficient and Fe-rich conditions. Axenic cultures showed that both plant and bacterium modified VOC synthesis in the presence of the respective symbiotic partner. Importantly, in both Fe-rich and -deficient experiments, bacterial VOCs increased the generation of plant biomass, rhizosphere acidification, ferric reductase activity, and chlorophyll content in plants. On the basis of our results, we propose that M. truncatula perceives its symbiont through VOC emissions, and in response, increases Fe-uptake mechanisms to facilitate symbiosis.

• MeSH
• biomasa MeSH
• chlorofyl analýza   MeSH
• FMN-reduktasa metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• kořenové hlízky rostlin mikrobiologie   MeSH
• Medicago truncatula chemie   růst a vývoj   metabolismus   mikrobiologie   MeSH
• půda chemie   MeSH
• Sinorhizobium meliloti metabolismus   MeSH
• těkavé organické sloučeniny metabolismus   MeSH
• železo metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH