The most frequently used method for establishing epidemiological relationships between Plesiomonas shigelloides strains is O:H serotyping. However, a number of strains are not serotypeable and isolates from diverse sources can display the same serovar. Moreover, since the zoonotic nature of Plesiomonas has been suggested and this hypothesis is based on the identical serovars found in animals and humans, we intend to use four DNA-based techniques: random amplified polymorphic DNA-PCR, enterobacterial repetitive intergenic consensus-PCR, repetitive extragenic palindromic-PCR, and pulsed field gel electrophoresis in order to screen 24 strains belonging to nine O:H serovars isolated from humans, animals, and the environment. In general, P. shigelloides showed a high genetic heterogeneity. Three pairs of strains, each containing a human and an animal isolate, displayed similar genotypes. This is the first report that provides molecular evidence that P. shigelloides may be zoonotic.
- MeSH
- genetická variace MeSH
- gramnegativní bakteriální infekce mikrobiologie přenos veterinární MeSH
- intergenová DNA MeSH
- lidé MeSH
- mikrobiologie životního prostředí MeSH
- molekulární typizace metody MeSH
- obrácené repetice MeSH
- Plesiomonas klasifikace genetika izolace a purifikace MeSH
- pulzní gelová elektroforéza metody MeSH
- repetitivní sekvence nukleových kyselin MeSH
- technika náhodné amplifikace polymorfní DNA metody MeSH
- zoonózy mikrobiologie přenos MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
A total of 73 strains of Plesiomonas shigelloides isolated from humans (24 strains) animals (21 strains) and aquatic environment (28 strains) were determined for their O:H serotype and susceptibility to 18 anti-microbial substances and to the vibriostatic agent O/129. Of all strains, 86.3% were typeable by the O and 94.5% by the H anti-sera used. The serotype distribution was heterogeneous within a country and between the countries. Of the 57 different serotypes identified, O11:H2 (2 strains), O22:H3 (4 strains), O35:HH11 (2 strains), O52:H3 (2 strains) and O90:H6 (2 strains) were found among isolates from humans and animals (mainly in cats) in Finland and Cuba, and O23:H1a1b (3 strains) among isolates from environmental sources in Slovak Republic and Italy. Most (93-100%) of all strains were susceptible to all anti-microbials tested but resistant (92-96%) to the broad-spectrum penicillins (ampicillin, mezlocillin). No correlation between anti-microbial resistance patterns and serotypes was found.
- MeSH
- antibakteriální látky farmakologie MeSH
- gramnegativní bakteriální infekce mikrobiologie MeSH
- kočky MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mikrobiologie vody * MeSH
- nemoci koček mikrobiologie MeSH
- O-antigeny izolace a purifikace MeSH
- Plesiomonas klasifikace účinky léků růst a vývoj MeSH
- sérotypizace MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Finsko MeSH
- Kuba MeSH