The 16S rRNA sequence analysis and biochemical characteristics were confirmed that the isolated bacterium is Pseudomonas sp. LBC1. The commonly used textile dye, Direct Brown MR has been used to study the fate of biodegradation. Pseudomonas sp. LBC1 showed 90% decolorization of Direct Brown MR (100 mg/L) and textile industry effluent with significant reduction in COD and BOD. The optimum condition for decolorization was 7.0 pH and 40°C. Significant increase in a activity of extracellular laccase suggested their possible involvement in decolorization of Direct Brown MR. Biodegradation metabolites viz. 3,6-dihydroxy benzoic acid, 2-hydroxy-7-aminonaphthol-3-sulfonic acid, and p-dihydroperoxybenzene were identified on the basis of mass spectra and using the 1.10 beta Shimadzu NIST GC-MS library. The Direct Brown MR and textile industry effluent were toxic to Sorghum bicolor and Vigna radiata plants as compared to metabolites obtained after decolorization. The Pseudomonas sp. LBC1 could be useful strain for decolorization and detoxification of textile dyes as well as textile industry effluent.
- MeSH
- barvicí látky metabolismus toxicita MeSH
- biodegradace MeSH
- koncentrace vodíkových iontů MeSH
- měření biologické spotřeby kyslíku MeSH
- molekulární sekvence - údaje MeSH
- odpadní vody chemie mikrobiologie MeSH
- průmyslový odpad škodlivé účinky analýza MeSH
- Pseudomonas genetika izolace a purifikace metabolismus MeSH
- půdní mikrobiologie MeSH
- regenerace a remediace životního prostředí metody MeSH
- Sorghum účinky léků MeSH
- textilní průmysl MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
