The crystal structure of an efficient Diels-Alder antibody catalyst at 1.9 angstrom resolution reveals almost perfect shape complementarity with its transition state analog. Comparison with highly related progesterone and Diels-Alderase antibodies that arose from the same primordial germ line template shows the relatively subtle mutational steps that were able to evolve both structural complementarity and catalytic efficiency.
- MeSH
- chemické jevy MeSH
- fyzikální chemie MeSH
- genetické matrice MeSH
- hapteny chemie metabolismus MeSH
- imunoglobuliny - Fab fragmenty chemie metabolismus MeSH
- katalytické protilátky chemie genetika metabolismus MeSH
- katalýza MeSH
- konformace proteinů MeSH
- krystalografie rentgenová MeSH
- ligandy MeSH
- molekulární evoluce * MeSH
- molekulární modely MeSH
- mutace MeSH
- progesteron imunologie MeSH
- rozpustnost MeSH
- teplota MeSH
- vazebná místa protilátek MeSH
- vodíková vazba MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
Current topics in microbiology and immunology, ISSN 0070-217X 139
VI, 152 s. : tab., grafy ; 26 cm
