The aim of this study was to investigate the tissue perfusion status and circadian rhythm in moderately premature infants. As a prospective study, from July 2019 to October 2019, the haemodynamic stability of moderate to late preterm, including such indicators as perfusion index (PI), blood pressure (systolic/diastolic) (BP), heart rate (HR), respiratory rate (RR), oxygen saturation (SpO2) and body temperature were monitored in the morning and at night within eight days after birth. There was no difference of statistical significance between PI values in the morning and at night (P>0.05). The HR from days six to eight after birth was higher than days one to three (P<0.05). The HR increased significantly on days seven and eight compared with days four and five (P<0.05). The BP from days three to eight was significantly higher than on day one (P<0.05), and the BP from days four to eight was higher than on day two. There was a weak positive correlation between the PI values and gestational age (GA) (r=0.097), HR (r=0.067) and time (r=0.284), and a negative correlation with SpO2 (r=-0.113). The PI and HR of moderate to late preterm increased within eight days after birth. BP was relatively lower after birth and gradually increased to a stable level on days three to four. The PI and BP circadian rhythms associated with tissue perfusion were not established on day eight after birth.
- MeSH
- krevní tlak fyziologie MeSH
- lidé MeSH
- novorozenec nedonošený * fyziologie MeSH
- novorozenec MeSH
- perfuze MeSH
- prospektivní studie MeSH
- srdeční frekvence fyziologie MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
Oxidative stress and NF-κB signaling plays a major role in pathogenesis of osteoarthritis. In the present study, we analyzed the potent role of carnosol against osteoarthritis in cells treated using monosodium iodoacetate (MIA) model through in vitro studies. MIA caused dose-dependent cell death and induced programmed cell death by increasing subG1 accumulation and caspase-3 expressions. MIA caused oxidative stress by increasing reactive oxygen species, lipid peroxidation and further induced NF-κB expression and down regulated Nrf-2 levels. Pre-treatment with carnosol significantly protected the cells by reducing the oxidative stress markers and improved the cell viability up to 98%. Further, carnosol down regulated NF-κB nuclear expression with a concomitant increase in Nrf-2 nuclear localization and up regulated the nuclear Nrf-2 levels. Carnosol also inhibited MIA-induced subG1 accumulation and caspase-3 activation. This study demonstrates that, carnosol might act as potent antioxidant and regulate MIA-induced oxidative stress, NF-κB signaling and programmed cell death by up regulating the Nrf-2 levels.
- MeSH
- antioxidancia metabolismus MeSH
- apoptóza účinky léků MeSH
- chondrocyty patologie účinky léků MeSH
- chrupavka MeSH
- diterpeny abietanové farmakologie metabolismus terapeutické užití MeSH
- faktor 1 související s NF-E2 aplikace a dávkování MeSH
- jodacetáty farmakologie škodlivé účinky MeSH
- kultivované buňky MeSH
- NF-kappa B antagonisté a inhibitory MeSH
- osteoartróza * metabolismus patofyziologie MeSH
- oxidační stres účinky léků MeSH
- potkani Sprague-Dawley MeSH
- reaktivní formy kyslíku antagonisté a inhibitory MeSH
- zánět genetika chemicky indukované patofyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH