HtrA serine peptidase 3 (HTRA3) is an important reproduction-related gene. In this study, we cloned the full-length coding sequence of the porcine HTRA3 gene using RT-PCR. Sequence analysis of this gene revealed that the pig HTRA3 gene encodes a protein of 453 amino acids, which has high homology with the HTRA3 protein of five species: water buffalo (96 %), killer whale (96 %), sperm whale (96 %), Yangtze River dolphin (96 %) and smalleared galago (93 %). Phylogenetic analysis revealed that the pig HTRA3 gene has a closer genetic relationship with the HTRA3 gene of cattle. PCR-HpaIIRFLP was established to detect the GU373693:c.1140 G>A substitution of the porcine HTRA3 gene coding sequence, and eight pig breeds displayed obvious genotype and allele frequency differences at this mutation locus. Association of this SNP with litter size traits was assessed in Large White (N = 200) and Landrace (N = 200) pig populations, and the results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White and Landrace sows (P < 0.05). Therefore, the HTRA3 gene could be a useful candidate for increasing the litter size in pigs. These data serve as a foundation for further insight into this novel porcine gene.
- MeSH
- frekvence genu MeSH
- fylogeneze MeSH
- genotyp MeSH
- jednonukleotidový polymorfismus genetika MeSH
- klonování DNA MeSH
- polymerázová řetězová reakce MeSH
- prasata MeSH
- sekvenční analýza DNA MeSH
- serinové endopeptidasy genetika metabolismus MeSH
- skot MeSH
- velikost vrhu MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The complete coding sequence of the porcine IBP4 gene was isolated using RT-PCR. Sequence analysis showed that the porcine IBP4 gene encodes a protein of 259 amino acids which shares high homology with the insulin-like growth factor binding protein 4 (IBP4) of eight species: cattle (97 %), goat (97 %), chimpanzee (97 %), human (96 %), giant panda (96 %), sheep (95 %), Sumatran orangutan (95 %) and rabbit (93 %). This gene is structured in four exons and three introns as revealed by computer- assisted analysis. Phylogenetic analysis revealed that the porcine IBP4 gene has a close genetic relationship with the IBP4 gene of cattle. Polymorphism analysis indicated that there was an A/G substitution in the position of 134 bp of exon 2 and this mutation did not alter the encoded amino acids of the porcine IBP4 gene. PCR-Hha I-RFLP revealed that eight pig breeds displayed obvious genotype and allele frequency differences at this polymorphic locus. Association of this SNP with litter size traits was assessed in Large White (N = 100) and Landrace (N = 100) populations, and the results demonstrated that this polymorphic locus was significantly associated with the litter size of the first parity in Large White sows and Landrace sows (P <0.05). These data serve as a foundation for further insight into this novel porcine gene.
- MeSH
- chov MeSH
- frekvence genu MeSH
- fylogeneze MeSH
- genetické asociační studie * MeSH
- genetické lokusy genetika MeSH
- genotyp MeSH
- komplementární DNA genetika MeSH
- kvantitativní znak dědičný MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- otevřené čtecí rámce genetika MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- polymorfismus délky restrikčních fragmentů genetika MeSH
- protein 4 vázající insulinu podobné růstové faktory chemie genetika metabolismus MeSH
- restrikční endonukleasy typu II metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- sekvenční seřazení MeSH
- Sus scrofa genetika MeSH
- velikost vrhu genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
