A terrestrial green microalga was isolated at Ås, in Akershus County, Norway. The strain corresponded to a coccoid chlorophyte. Morphological characteristics by light and electron microscopy, in conjunction with DNA amplification and sequencing of the 18 s rDNA gene and ITS sequences, were used to identify the microalgae. The characteristics agree with those of the genus Coelastrella defined by Chodat, and formed a sister group with the recently described C. thermophila var. globulina. Coelastrella is a relatively small numbered genus that has not been observed in continental Norway before; there are no previous cultures available in collections of Norwegian strains. Gas chromatography analyses of the FAME-derivatives showed a high percentage of polyunsaturated fatty acids (44-45%) especially linolenic acid (C18:3n3; 30-34%). After the stationary phase, the cultures were able to accumulate several carotenoids as neoxanthin, pheophytin a, astaxanthin, canthaxanthin, lutein, and violaxanthin. Due to the scarcity of visual characters suitable for diagnostic purposes and the lack of DNA sequence information, there is a high possibility that species of this genus have been neglected in local environmental studies, even though it showed interesting properties for algal biotechnology.
- MeSH
- biologické pigmenty analýza MeSH
- biotechnologie MeSH
- Chlorophyta klasifikace cytologie genetika MeSH
- druhová specificita MeSH
- feofytiny analýza MeSH
- fylogeneze * MeSH
- karotenoidy analýza MeSH
- kyselina alfa-linolenová analýza MeSH
- mastné kyseliny analýza MeSH
- mikrořasy klasifikace cytologie genetika izolace a purifikace MeSH
- ribozomální DNA MeSH
- RNA ribozomální 18S genetika MeSH
- xanthofyly MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Norsko MeSH
Nutritional factors which exhibit antioxidant properties, such as those contained in green plants, may be protective against cancer. Chlorophyll and other tetrapyrrolic compounds which are structurally related to heme and bilirubin (a bile pigment with antioxidant activity) are among those molecules which are purportedly responsible for these effects. Therefore, the aim of our study was to assess both the antiproliferative and antioxidative effects of chlorophylls (chlorophyll a/b, chlorophyllin, and pheophytin a) in experimental pancreatic cancer. Chlorophylls have been shown to produce antiproliferative effects in pancreatic cancer cell lines (PaTu-8902, MiaPaCa-2, and BxPC-3) in a dose-dependent manner (10-125 μmol/L). Chlorophylls also have been observed to inhibit heme oxygenase (HMOX) mRNA expression and HMOX enzymatic activity, substantially affecting the redox environment of pancreatic cancer cells, including the production of mitochondrial/whole-cell reactive oxygen species, and alter the ratio of reduced-to-oxidized glutathione. Importantly, chlorophyll-mediated suppression of pancreatic cancer cell viability has been replicated in in vivo experiments, where the administration of chlorophyll a resulted in the significant reduction of pancreatic tumor size in xenotransplanted nude mice. In conclusion, this data suggests that chlorophyll-mediated changes on the redox status of pancreatic cancer cells might be responsible for their antiproliferative and anticancer effects and thus contribute to the decreased incidence of cancer among individuals who consume green vegetables.
- MeSH
- antioxidancia metabolismus MeSH
- chlorofyl farmakologie MeSH
- extracelulárním signálem regulované MAP kinasy metabolismus MeSH
- feofytiny metabolismus MeSH
- glutathion metabolismus MeSH
- glutathiondisulfid metabolismus MeSH
- hemová oxygenasa (decyklizující) metabolismus MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- mitochondrie účinky léků metabolismus MeSH
- nádorové buněčné linie MeSH
- nádory slinivky břišní metabolismus MeSH
- oxidace-redukce účinky léků MeSH
- peroxid vodíku metabolismus MeSH
- protinádorové látky farmakologie MeSH
- superoxidy metabolismus MeSH
- Synechocystis chemie MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
