Úvod a cíle: Laminarin je nízkomolekulární (504 Da) větvený glukózový polysacharid [ (1®3) -b-D-glukan], který je schopen modulovat humorální a buněčnou imunitní odpověď, a to nespecifickou i specifickou. Laminarin se vyznačuje anti-tumorózní aktivitou, neboť indukuje apoptózu a ovlivňuje intestinální mikrobiota. Cílem studie bylo vyšetřit anti-laminarinové protilátky u pacientů s kolorektálním karcinomem. Metody: Do prospektivní studie bylo zahrnuto 46 jedinců, z toho 14 kontrol (5 mužů, 9 žen, věk 29–80, průměr 55 ± 13) a 32 pacientů s kolorektálním karcinomem (14 mužů, 18 žen, věk 46–86, průměr 66 ± 11). Dva pacienti z CRC skupiny byli vyřazeni pro zjištěnou odlehlou hodnotu. Z 30 pacientů s CRC mělo 12 pravostranný kolorektální karcinom (12/30, 40 %). Většina pacientů s CRC měla stadium III (13/30, 43 %) nebo IV (13/30, 43 %). Pacienti měli převážně středně diferencovaný (15/30, 50 %) nebo nízce diferencovaný (11/30, 37 %) karcinom. Vzorky byly odebírány z periferní žilní krve a sérové IgG protilátky proti laminarinu byly stanoveny metodou ELISA v jednotkách U/ml. Výsledky: Sérové protilátky proti laminarinu byly signifikantně vyšší u kontrol ve srovnání s pacienty s CRC (16,23 ± 6,60; 11,41 ± 5,53; p = 0,015) a u kontrol ve srovnání s pacienty s levostranným CRC (11,38 ± 5,39; p = 0,046). Významný rozdíl byl pozorován mezi kontrolami a CRC III. stadiem (11,01 ± 3,36; p = 0,017); mezi kontrolami a CRC IV. stadiem (10,98 ± 6,06; p = 0,049). Anti-laminarinové protilátky byly signifikantně nižší u středně diferencovaného CRC ve srovnání s kontrolami (10,78 ± 5,22; p = 0,020), avšak nikoli u nízce diferencovaného CRC (12,10 ± 6,28; p = 0,755). Nebyl prokázán rozdíl mezi kontrolami a ženami s CRC (11,94 ± 6,39; p = 0,092). Byl potvrzen signifikantní rozdíl mezi kontrolami a muži s CRC (10,72 ± 4,32; p = 0,017). Závěr: Sérové protilátky proti laminarinu byly signifikantně nižší u pacientů s CRC a u podskupin osob s kolorektálním karcinomem.
Introduction and objectives: Laminarin is a low molecular weight (504 Da) glucose branched polysaccharide which modulates humoral and cellular immune response, both non-specific and specific. Laminarin possesses anti-tumorous activity as it induces apoptosis and modulates the colonic microbiota. The aim of our current study was to evaluate anti-laminarin antibodies in colorectal carcinoma. Methods: A total of 46 individuals were enrolled in the prospective study including 14 controls (5 men, 9 women, age 29–80, mean 55 ± 13) and 32 patients with colorectal carcinoma, CRC (14 men, 18 women, age 46–86, mean 66 ± 11). Two outliers were identified in the CRC group. Out of 30 CRC patients, 12 individuals had right-sided CRC (12/30; 40%). Majority of the CRC patients had stage III (13/30; 43%) or IV (13/30; 43%) cancer. Most of the CRC patients had moderately (15/30; 50%) or poorly (11/30; 37%) differentiated CRC. Samples were obtained from the peripheral venous blood and investigation of the serum IgG anti-laminarin antibodies was performed by means of ELISA and measured in U/mL. Results: Serum anti-laminarin antibodies were significantly higher in controls compared to the CRC group (16.23 ± 6.60; 11.41 ± 5.53; p = 0.015) and in controls compared to left-sided carcinoma (11.38 ± 5.39; p = 0.046). A statistically significant difference was observed between controls and CRC stage III (11.01 ± 3.36; p = 0.017) and between controls and CRC stage IV (10.98 ± 6.06; p = 0.049). Anti-laminarin antibodies were significantly lower in moderately differentiated CRC compared to controls (10.78 ± 5.22; p = 0.020), but not in poorly differentiated CRC (12.10 ± 6.28; p = 0.755). No difference was identified between controls and females with CRC (11.94 ± 6.39; p = 0.092). There was a significant difference between controls and males with CRC (10.72 ± 4.32; p = 0.017). Conclusion: Serum anti-laminarin antibodies were significantly lower in the CRC group and CRC subgroups compared to controls.
- Klíčová slova
- anti-laminarinové protilátky,
- MeSH
- celulasy analýza MeSH
- kolorektální nádory * patofyziologie MeSH
- lidé MeSH
- nádorové biomarkery MeSH
- prospektivní studie MeSH
- Check Tag
- lidé MeSH
Liquid cultures with cellulose and solid state fermentation cultures on wheat straw of the white-rot fungi Pleurotus ostreatus and Trametes versicolor and the brown-rot fungus Piptoporus betulinus were assayed for the free and solid fraction-bound activity of lignocellulose-degrading enzymes. The majority of the ligninolytic enzymes laccase and Mn peroxidase was detected in the free fraction of P. ostreatus and T. versicolor. The endocleaving enzymes endo-1,4-beta-glucanase, endo-1,4-beta-mannanase and endo-1,4-beta-xylanase were detected almost exclusively in the free fraction, while significant amounts of 1,4-beta-glucosidase, cellobiohydrolase, 1,4-beta-xylosidase and 1,4-beta-mannosidase were present in the bound fraction depending on the mode of cultivation and the species. The bound enzymes accounted for 66% of the total activity in P. ostreatus straw cultures, 35% in T. versicolor and only 8% in P. betulinus. The enzymes also showed significant differences in freeze-drying stability. Hydrolases in general showed high stability, whereas laccase and Mn peroxidase of P. ostreatus were the least stable.
- MeSH
- celulasy analýza MeSH
- celulosa metabolismus MeSH
- enzymy analýza MeSH
- financování organizované MeSH
- fungální proteiny analýza MeSH
- glykosidhydrolasy analýza MeSH
- kultivační média chemie MeSH
- lakasa analýza MeSH
- lignin metabolismus MeSH
- lyofilizace MeSH
- peroxidasy analýza MeSH
- Pleurotus enzymologie MeSH
- Polyporales enzymologie MeSH
- stabilita enzymů MeSH
