The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.
- MeSH
- analýza jednotlivých buněk MeSH
- buněčná diferenciace genetika MeSH
- buněčný rodokmen genetika MeSH
- epitelové buňky metabolismus MeSH
- hematoencefalická bariéra metabolismus MeSH
- mozek metabolismus fyziologie MeSH
- myši inbrední C57BL MeSH
- myši embryologie MeSH
- nemoci mozku genetika patofyziologie MeSH
- plexus chorioideus embryologie metabolismus fyziologie MeSH
- signální transdukce MeSH
- stárnutí fyziologie MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši embryologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- MeSH
- hydrokortison MeSH
- kuřecí embryo MeSH
- plexus chorioideus embryologie účinky léků MeSH
- Check Tag
- kuřecí embryo MeSH
