Utilization of relatively low-cost modification of Fenton reaction for the elimination of selected antibiotics and resistant coliforms in different part of wastewater treatment plant (WWTP) was studied. The concentration of antibiotics and occurrence of resistant gems in different stages of WWTP in the capital city of Slovakia - Bratislava was analyzed by LC-MS/MS technique. Consequently, Fenton-like reaction was applied for the elimination of chemical and biological contaminants. Comparative study with classical Fenton reaction was also done. Very high concentrations of clarithromycin, ciprofloxacin and azithromycin in influent water were found. Coliform bacteria were predominantly resistant to ampicillin, ciprofloxacin and gentamicin. After the mechanical stage, the concentration of antibiotics in water was significantly decreased because of the sorption during this step. Biological step degraded 12 types of antibiotics. Analyses of effluent water showed very bad elimination of azithromycin (919ng/L) and clarithromycin (684ng/L). Contrary, ciprofloxacin was removed with very high efficiency (95%). The number of resistant bacteria was also significantly decreased in effluent water. In the case of Escherichia coli only ampicillin and gentamicin resistance bacteria were detected. Our results show that antibiotics as well as resistant bacteria were eliminated by the modification of classical Fenton reaction with high efficiency. The modification of the Fenton reaction can decrease the process wages, environmental impact. Moreover, the degradation process was easily controlled, monitored and tuned.
- MeSH
- ampicilin analýza MeSH
- antibakteriální látky analýza MeSH
- bakteriální léková rezistence MeSH
- chemické látky znečišťující vodu analýza MeSH
- ciprofloxacin analýza MeSH
- čištění vody metody MeSH
- gentamiciny analýza MeSH
- mikrobiologie vody * MeSH
- odpadní voda analýza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Slovenská republika MeSH
Bujónovou diluční mikrometodou a standardní diskovou difuzní metodou EUCAST byla vyšetřena citlivost k ciprofloxacinu, tetracyklinu a erytromycinu u konsekutivních klinických izolátů Campylobacter jejuni (n = 693) a C. coli (n = 75), zaslaných 49 mikrobiologickými laboratořemi v průběhu jednoho měsíce do Národní referenční laboratoře pro antibiotika SZÚ. Při interpretaci výsledků, získaných z 4608 měření kombinací kmen-antibiotikum dvěma metodami a interpretovaných podle klinických breakpointů EUCAST se vyskytly pouze dvě diskrepance (0,043 %) mezi hodnotou minimální inhibice a příslušným průměrem inhibiční zóny. Lze konstatovat, že při pečlivém dodržení postupu vyšetření je standardní disková difuzní metoda EUCAST pro vyšetření antibiotické citlivosti Campylobacter jejuni a C. coli vysoce reprodukovatelná.
The EUCAST broth dilution micromethod and standard disk diffusion method were used to test susceptibility to ciprofloxacin, tetracycline, and erythromycin in consecutive clinical isolates of Campylobacter jejuni (n=693) and Campylobacter coli (n=75) referred to the National Reference Laboratory for Antibiotics of the National Institute of Public Health from 49 microbiological laboratories within one month. When considering the results obtained in 4608 measurements of strain-antimicrobial combinations by two methods and interpreted according to the EUCAST clinical breakpoints, only two discrepancies (0.043%) were found between the value of the minimal inhibitory concentration and the respective inhibition zone diameter. It can be stated that as long as the protocol is followed carefully, the EUCAST standard disk diffusion method for antimicrobial susceptibility testing of Campylobacter jejuni and Campylobacter coli shows high reproducibility.
- MeSH
- bakteriální léková rezistence * MeSH
- bakteriologické techniky MeSH
- Campylobacter coli * patogenita účinky léků MeSH
- Campylobacter jejuni * patogenita účinky léků MeSH
- ciprofloxacin analýza MeSH
- erythromycin analýza MeSH
- kampylobakterové infekce * farmakoterapie MeSH
- lidé MeSH
- mikrobiální testy citlivosti metody normy trendy MeSH
- tetracyklin analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- tabulky MeSH
Until now, analysis of Ofloxacin in human blood plasma using solid-phase extraction (SPE) by HPLC UV detector has not been reported. This study aims to determine the validity of analytical methods in Ofloxacin study in human blood plasma (in vitro) using an HPLC SPE UV detector. Plasma samples were extracted by SPE. Analytes were analyzed using a C18 column (octadecylsilane) 250x4.6 mm, particle size 10 μm, mobile phase 85,5:14,5 v v 0.025 M phosphate buffer (pH 2.2) and acetonitrile with a flow rate of 2 ml/min, detection performed at 294 nm with the internal standard ciprofloxacin. Validated analytical method was based on the parameters: selectivity, accuracy, precision, repeatability, linearity, LOD, LOQ, and the suitability of the system. Validation analysis showed selectivity test Rs>1.5, test repeatability with CV(%) <10%, linearity was obtained in the range of 0.1 to 6 µg/ml with correlation coefficient (r) from 0.9998 to 0.9999. Based on the area ratio of peak height and a segment of the chromatogram obtained LOD values 0.023 and 0.024 µg/ml, LOQ value of 0.076 and 0.080 tg/ml, percent accuracy from 94.32 to 100.45% and 97.68 to 101.63%, and precision CV (%) 0.31 to 0.85% and 0.84 to 1.08%. System suitability test results on the retention time, area ratio, and high ratios of peak chromatogram shows the CV(%) <10%. Can be concluded that the analytical methods used have validity in accordance with the requirements.
- MeSH
- antibakteriální látky izolace a purifikace MeSH
- ciprofloxacin * analýza izolace a purifikace krev MeSH
- extrakce na pevné fázi * metody přístrojové vybavení MeSH
- krevní plazma MeSH
- lidé MeSH
- ofloxacin * analýza izolace a purifikace krev MeSH
- vysokoúčinná kapalinová chromatografie * metody MeSH
- Check Tag
- lidé MeSH
