Turnip ringspot virus (TuRSV) has been proposed to be a member of a new species in the genus Comovirus. Its remarkable host-range similarity to radish mosaic virus (RaMV) may have led to its misrecognition in the past. Findings from both sequence analysis and serological tests support the assignment of TuRSV to a new comovirus species. In addition, phylogenetic analysis suggests that the two genome segments of some TuRSV isolates have a heterogeneous origin.
- MeSH
- Brassica napus MeSH
- Comovirus klasifikace genetika imunologie izolace a purifikace MeSH
- fylogeneze MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- Raphanus MeSH
- RNA virová genetika MeSH
- sekvenční analýza DNA MeSH
- sérotypizace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The complete genome sequence of a severe isolate of broad bean true mosaic virus (genus Comovirus, subfamily Comovirinae, family Secoviridae) is presented. Comparison of the amino acid sequences of the capsid proteins and the polymerase showed striking differences to other comoviruses and highest similarities to legume-infecting comoviruses. Red clover mottle virus was recognized as the most similar virus with amino acid sequence identities ranging from 43 to 67% for individual genes.
The intraspecies variability of capsid proteins of five viruses of the genus Comovirus was established. Inclusion of both capsid proteins to the sequence analysis reduces some uncertainties about species/strains demarcation criteria in the Comovirus genus. New approach is proposed for discrimination of Turnip ringspot virus as a separate species.
