DNA repair events have functional significance especially for genome stability. Although the DNA damage response within the whole genome has been extensively studied, the region-specific characteristics of nuclear sub-compartments such as the nucleolus or fragile sites have not been fully elucidated. Here, we show that the heterochromatin protein HP1 and PML protein recognize spontaneously occurring 53BP1- or γ-H2AX-positive DNA lesions throughout the genome. Moreover, 53BP1 nuclear bodies, which co-localize with PML bodies, also occur within the nucleoli compartments. Irradiation of the human osteosarcoma cell line U2OS with γ-rays increases the degree of co-localization between 53BP1 and PML bodies throughout the genome; however, the 53BP1 protein is less abundant in chromatin of ribosomal genes and fragile sites (FRA3B and FRA16D) in γ-irradiated cells. Most epigenomic marks on ribosomal genes and fragile sites are relatively stable in both non-irradiated and γ-irradiated cells. However, H3K4me2, H3K9me3, H3K27me3 and H3K79me1 were significantly changed in promoter and coding regions of ribosomal genes after exposure of cells to γ-rays. In fragile sites, γ-irradiation induces a decrease in H3K4me3, changes the levels of HP1β, and modifies the levels of H3K9 acetylation, while the level of H3K9me3 was relatively stable. In these studies, we confirm a specific DNA-damage response that differs between the ribosomal genes and fragile sites, which indicates the region-specificity of DNA repair.

• MeSH
• chromatin genetika   MeSH
• chromozomální proteiny, nehistonové metabolismus   účinky záření   MeSH
• DNA vazebné proteiny účinky záření   MeSH
• fibroblasty účinky záření   MeSH
• fragilní místa na chromozomu genetika   MeSH
• histony účinky záření   MeSH
• jaderné proteiny metabolismus   účinky záření   MeSH
• lidé MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nádorové supresorové proteiny metabolismus   účinky záření   MeSH
• nestabilita genomu MeSH
• oprava DNA genetika   MeSH
• osteosarkom MeSH
• poškození DNA účinky záření   MeSH
• ribozomy genetika   MeSH
• transkripční faktory metabolismus   účinky záření   MeSH
• záření gama MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH