Huminové látky představují podstatu chemického účinku peloidních koupelí, tedy koupelí v rašelině, slatině, případně v jejich extraktech. Tento chemický účinek se blahodárně spojuje s účinkem flotačním, kompresivním a termickým. Zda a případně v jakém stupni dochází k přechodu huminových látek do těla pacienta, je předmětem léta trvající diskuse. V této souvislosti se zdůrazňuje význam teploty aplikační koupele, stupně ionizace a aktuálního pH. V jednoduchém uspořádání jsme sledovali chování huminových látek při expozici pacienta celotělové koupeli. Koncentrace huminových látek v koupeli byla sledována po přidání 100 ml vodného extraktu s obsahem 54 g/l do vany s teplou vodou (38 °C), a to bez pacienta/klienta a s pacientem/klientem. Vzorky 250 ml byly odebrány v časech 0 = start, 15 min., 20 min. a 30 min. Po provedení korekce koncentrací na evaporaci byly srovnány koncentrace huminových látek ve vzorcích z vany bez pacienta/klienta a s pacientem/klientem. Získané rozdíly koncentrací byly přepočteny na 1 m2 tělesného povrchu exponované osoby. Výsledky vedou k předpokladu, že ve zvoleném uspořádání lze očekávat pokles koncentrací (a tedy pravděpodobnou extrakci do kůže exponované osoby) o 4 mg/m2 povrchu těla exponované osoby. Množstevně vyjádřeno jde o minimálně 477,27 mg/m2 povrchu těla exponované osoby.
Humic substances represent the essence of the chemical impact of peloid baths, e.g. baths in peat and mud, and eventually in their extracts. This chemical effect is beneficially associated with flotation, compressive and thermic effects. Whether, and at which level the transition of humic substances has an effect on the patient's body, has been a subject of an ongoing debate for years. In this context, the importance of the application of the bath temperature, the degree of ionization and current pH are all highlighted. In a simple formula, we have followed the behaviour of humic substances to the the patient's exposure to the whole-body bath. The concentration of humic substances in the bath was monitored after the addition of 100 ml of aqueous extract containing 54 g/l placed into the bath with warm water (38 °C), with or without a patient/client. 250 ml samples were taken at the following time intervals 0 = start, 15 min., 20 min. and 30 min. After making adjustments for evaporation, the concentrations of humic substances in samples from the baths with or without a patient/client, were compared. The concentration differences were recalculated per 1 m2 of the body surface of the exposed person. The results lead us to the assumption that in a selected system it can be expected that the concentrations decrease (and therefore likely extraction to the skin of an exposed person) by 4 mg/m2 of the body surface of the exposed person. Expressed quantitatively, it is a minimum of 477.27 mg/m2 of the body surface of an exposed person.
- Klíčová slova
- peloidy, peloidní extrakty, transfer huminových látek do organismu,
- MeSH
- fyzikální absorpce MeSH
- huminové látky * škodlivé účinky toxicita využití MeSH
- lázně MeSH
- lidé MeSH
- terapie bahnem * MeSH
- Check Tag
- lidé MeSH
Humic substances (HS) are ubiquitous natural products of decomposition of dead organic matter. HS is present in most freshwaters at concentrations ranging from 0.5 to 50mg L(-1). Organic carbon can represent 20% dry weight of sediments. Recently, the interaction of dissolved HS with the aryl hydrocarbon receptor (AhR) has been demonstrated. The AhR is a cytosolic receptor to which persistent organic pollutants (POPs) can bind and many of their toxic effects are mediated through interactions with this receptor. We describe in vitro effects (using H4IIE-luc cells) of binary mixtures of various HS with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), since in contaminated environments these compounds occur simultaneously. Six out of 12 HS samples activated AhR even at environmentally relevant concentrations (17 mg L(-1)), but did not reach the full AhR-activation even at excessive concentration. In simultaneous exposure of H4IIE-luc to HS (17 mg L(-1)) and TCDD (1.2 pM) without any preincubation prior to exposure, either significant additive or facilitative effects were observed. No negative interactions, due to possible sorption of TCDD to HS was observed. Nevertheless, if the HS-TCDD binary mixture was preincubated for 6 days prior to the exposure on H4IIE-luc cells, the additive and facilitative effects were less due to possible sorption of TCDD onto HS. Similar results were obtained from analogous experiments with greater concentrations of both TCDD and HS.
- MeSH
- huminové látky toxicita MeSH
- kinetika MeSH
- krysa rodu rattus MeSH
- látky znečišťující půdu toxicita MeSH
- nádorové buněčné linie MeSH
- polychlorované dibenzodioxiny toxicita MeSH
- receptory aromatických uhlovodíků metabolismus MeSH
- regenerace a remediace životního prostředí metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10(-8)-7.4 x 10(-8). Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists. Contribution of persistent organic compounds to the observed effects was ruled out by H(2)SO(4) treatment. Eight out of twelve HS elicited significant antiestrogenic effects with IC(50) ranging from 40 to 164 mg l(-1). The possible explanations of the antiestrogenic effect include sorption of 17-beta-estradiol (E2) on HS, changes in membrane permeability for E2 or another specific mechanism.
- MeSH
- benzopyrany toxicita MeSH
- financování organizované MeSH
- huminové látky toxicita MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- modulátory estrogenních receptorů farmakologie MeSH
- nádorové buněčné linie MeSH
- polychlorované dibenzodioxiny toxicita MeSH
- receptory aromatických uhlovodíků fyziologie účinky léků MeSH
- receptory pro estrogeny účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- MeSH
- histologie MeSH
- huminové látky toxicita MeSH
- krysa rodu rattus MeSH
- LD50 MeSH
- myši MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
