Neuronal activity in the medulla oblongata and neurogenic inflammation of airways were investigated in a guinea pig model induced by repeated intra-esophageal instillation of hydrochloric acid (HCl) after vagotomy. Unilateral vagotomy was performed in the vagotomy group, while a sham-operation was performed in the sham group. Operation was not conducted in sham control group. Airway inflammation was observed with hematoxylin and eosin (HE) staining. C-fos protein was measured by immunohistochemistry (IHC) and Western blot (WB). Substance P was examined by IHC and enzyme-linked immuno sorbent assay (ELISA). Airway microvascular permeability was detected by evans blue dye (EBD) fluorescence. Inflammation of airway was observed in the trachea and bronchi after chronic HCl perfusion into the lower esophagus, and was alleviated after unilateral vagotomy. C-fos expression in the medulla oblongata was lower in the vagotomy group compared to the sham control and sham groups. Substance P-like immunoreactivity (SP-li), concentration and microvascular leakage in airway were lower in the vagotomy group than that in the other groups. Our results suggest that vagotomy improved neurogenic inflammation of airways and decreased neuronal activities, the afferent nerves and neurons in medulla oblongata may be involved in neurogenic inflammation of airways mediated by esophageal-bronchial reflex.

• MeSH
• abnormální reflex MeSH
• aspirační pneumonie chemicky indukované   patofyziologie   chirurgie   MeSH
• ezofágus inervace   MeSH
• kapilární permeabilita MeSH
• kyselina chlorovodíková * MeSH
• medulla oblongata metabolismus   patofyziologie   MeSH
• morčata MeSH
• neurogenní zánět chemicky indukované   patofyziologie   chirurgie   MeSH
• plíce inervace   metabolismus   MeSH
• protoonkogenní proteiny c-fos metabolismus   MeSH
• substance P metabolismus   MeSH
• vagotomie * MeSH
• zvířata MeSH
• Check Tag
• morčata MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Transient receptor potential vanilloid (TRPV1) receptors are abundant in a subpopulation of primary sensory neurons that convey nociceptive information from the periphery to the spinal cord dorsal horn. The TRPV1 receptors are expressed on both the peripheral and central branches of these dorsal root ganglion (DRG) neurons and can be activated by capsaicin, heat, low pH, and also by recently described endogenous lipids. Using patch-clamp recordings from superficial dorsal horn (DH) neurons in acute spinal cord slices, the effect of application of the endogenous TRPV1 agonist N-oleoyldopamine (OLDA) on the frequency of miniature excitatory postsynaptic currents (mEPSCs) was evaluated. A high concentration OLDA (10 microM) solution was needed to increase the mEPSC frequency, whereas low concentration OLDA (0.2 microM) did not evoke any change under control conditions. The increase was blocked by the TRPV1 antagonists SB366791 or BCTC. Application of a low concentration of OLDA evoked an increase in mEPSC frequency after activation of protein kinase C by phorbol ester (PMA) and bradykinin or in slices from animals with peripheral inflammation. Increasing the bath temperature from 24 to 34 degrees C enhanced the basal mEPSC frequency, but the magnitude of changes in the mEPSC frequency induced by OLDA administration was similar at both temperatures. Our results suggest that presumed endogenous agonists of TRPV1 receptors, like OLDA, could have a considerable impact on synaptic transmission in the spinal cord, especially when TRPV1 receptors are sensitized. Spinal TRPV1 receptors could play a pivotal role in modulation of nociceptive signaling in inflammatory pain.

• MeSH
• analýza rozptylu MeSH
• anilidy farmakologie   MeSH
• bradykinin farmakologie   MeSH
• cinnamáty farmakologie   MeSH
• dopamin analogy a deriváty   farmakologie   MeSH
• excitační postsynaptické potenciály MeSH
• forbolové estery farmakologie   MeSH
• kationtové kanály TRPV agonisté   antagonisté a inhibitory   MeSH
• krysa rodu rattus MeSH
• měření bolesti metody   MeSH
• metoda terčíkového zámku metody   MeSH
• mícha cytologie   MeSH
• modely nemocí na zvířatech MeSH
• nervové receptory účinky léků   MeSH
• neurogenní zánět chemicky indukované   patofyziologie   MeSH
• novorozená zvířata MeSH
• potkani Wistar MeSH
• signální transdukce fyziologie   účinky léků   MeSH
• spinální ganglia cytologie   MeSH
• techniky in vitro MeSH
• teplota MeSH
• vazodilatancia farmakologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH