Terrestrial gastropod molluscs are widely distributed and are well known as pests of many types of plants that are notoriously difficult to control. Many species of nematodes are able to parasitize land snails and slugs, but few of them are lethal to their host. Species and/or populations of mollusc-parasitic nematodes (MPNs) that kill their hosts are promising for biological control purposes. The recent discovery of new nematode species of the genus Phasmarhabditis in Europe and the associations between Alloionema spp. and slugs are expanding the possibilities of using MPNs as control agents. However, very little is known about the distribution and ecology of these species. Using molecular techniques based on qPCR methods for quick identification and quantification of various species of MPN isolated directly from the soil or from infected hosts can assist in providing information on their presence and persistence, as well as the composition of natural assemblages. Here, we developed new primers and probes for five species of the genus Phasmarhabditis and one species of the genus Alloionema. We employed these novel molecular techniques and implemented a published molecular set to detect MPN presence in soil samples coming from natural and agricultural areas in Switzerland. We also developed a method that allows the detection and quantification of Phasmarhabditis hermaphrodita directly from the tissues of their slug host in a laboratory experiment. The new molecular approaches were optimized to a satisfactory limit of detection of the species, with only few cross-amplifications with closely related species in late cycles (>32). Using these tools, we detected MPNs in 7.5% of sampled sites, corresponding to forest areas (P. hermaphrodita and Alloionema appendiculatum) and wheat-oriented agricultural areas (Phasmarhabditis bohemica). Moreover, we confirmed that the method can be used to detect the presence of P. hermaphrodita inside slug hosts, with more detections in the susceptible slug Deroceras larvae compared to the resistant Arion vulgaris. These primers/probe sets provide a novel and quick tool to identify MPNs from soil samples and infected slugs without having to culture and retrieve all nematode life stages, as well as a new tool to unravel the ecology of nematode-slug complexes.

• MeSH
• biologická kontrola škůdců MeSH
• DNA helmintů genetika   MeSH
• hlemýždi parazitologie   MeSH
• hlístice genetika   izolace a purifikace   parazitologie   MeSH
• interakce hostitele a parazita MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• půda parazitologie   MeSH
• Rhabditoidea genetika   izolace a purifikace   parazitologie   MeSH
• ribozomální DNA genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Švýcarsko MeSH

A new nematode species, Rhabdias lacertae n. sp. (Rhabdiasidae), is described from the body-cavity of the common lizard Lacerta vivipara Jacquin (Lacertidae) from the Ridge of Malá Fatra (Sokolie Hill), north-western Slovakia. The new species differs from its congeners mainly in possessing 3 min cuticular spikes at the tail tip and some other features. This is the first species of Rhabdias Stiles & Hassall, 1905 described from lizards in Europe and the first species of this genus parasitising hosts belonging to the Lacertidae.

• MeSH
• infekce hlísticemi z řádu Rhabditida parazitologie   veterinární   MeSH
• ještěři parazitologie   MeSH
• Rhabditoidea anatomie a histologie   klasifikace   růst a vývoj   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH

• MeSH
• biologická kontrola škůdců metody   MeSH
• můry parazitologie   MeSH
• Rhabditoidea izolace a purifikace   patologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Geografické názvy
• Kanada MeSH