Studies of the human defensins have been hampered by the lack of a simple expression system allowing for rapid production of functional peptide forms. Here, we describe a Saccharomyces cerevisiae AH22 expression system that meets that condition. The 42 amino acid form of human beta-defensin-1 was expressed under the control of the ADH1 promoter. The optimum conditions for expression were determined and the stable maintenance of the pVT103L-hBD-1 chimeric vector in the yeast population was confirmed. Expressed hBD-1 was secreted into the medium (approximately 55 microg l(-1)) and purified using cation-exchange chromatography. Isolated defensin exhibited strong bactericidal effect on Escherichia coli ML-35p. We conclude that the expression system described here will be a useful tool where readily prepared and active forms of the human defensins are needed.
- MeSH
- antibakteriální látky biosyntéza farmakologie izolace a purifikace MeSH
- beta-defensiny biosyntéza farmakologie genetika chemie MeSH
- Escherichia coli cytologie účinky léků MeSH
- genetické vektory genetika MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- proliferace buněk účinky léků MeSH
- rekombinantní proteiny biosyntéza farmakologie chemie izolace a purifikace MeSH
- Saccharomyces cerevisiae genetika MeSH
- sekvence aminokyselin MeSH
- Check Tag
- lidé MeSH
