Understanding the ecology of coniferous forests is very important because these environments represent globally largest carbon sinks. Metatranscriptomics, microbial community and enzyme analyses were combined to describe the detailed role of microbial taxa in the functioning of the Picea abies-dominated coniferous forest soil in two contrasting seasons. These seasons were the summer, representing the peak of plant photosynthetic activity, and late winter, after an extended period with no photosynthate input. The results show that microbial communities were characterized by a high activity of fungi especially in litter where their contribution to microbial transcription was over 50%. Differences in abundance between summer and winter were recorded for 26-33% of bacterial genera and < 15% of fungal genera, but the transcript profiles of fungi, archaea and most bacterial phyla were significantly different among seasons. Further, the seasonal differences were larger in soil than in litter. Most importantly, fungal contribution to total microbial transcription in soil decreased from 33% in summer to 16% in winter. In particular, the activity of the abundant ectomycorrhizal fungi was reduced in winter, which indicates that plant photosynthetic production was likely one of the major drivers of changes in the functioning of microbial communities in this coniferous forest.

• MeSH
• Archaea klasifikace   genetika   MeSH
• Bacteria klasifikace   genetika   MeSH
• borovicovité mikrobiologie   MeSH
• ekosystém MeSH
• fotosyntéza MeSH
• genetická transkripce genetika   MeSH
• houby klasifikace   genetika   MeSH
• lesy MeSH
• messenger RNA biosyntéza   genetika   MeSH
• mikrobiota genetika   MeSH
• mykorhiza MeSH
• půda MeSH
• půdní mikrobiologie * MeSH
• roční období MeSH
• stanovení celkové genové exprese MeSH
• stromy mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Fungi from the genus Geosmithia (Ascomycota: Hypocreales) are associated with bark beetles (Coleoptera: Scolytinae), though little is known about ecology, diversity, and distribution of these fungi across beetle and its host tree species. This study surveyed the diversity, distribution and vector affinity of Geosmithia isolated from subcortical insects that colonized trees from the family Pinaceae in Central and Northeastern Europe. Twelve Geosmithia species were isolated from 85 plant samples associated with 23 subcortical insect species (including 14 bark beetle species). Geosmithia community composition was similar across different localities and vector species; although the fungal communities associated with insects that colonized Pinus differed from that colonizing other tree species (Abies, Larix, and Picea). Ten Geosmithia species from four independent phylogenetic lineages were not reported previously from vectors feeding on other plant families and seem to be restricted to the vectors from Pinaceae only. We conclude that presence of such substrate specificity suggests a long and stable association between Geosmithia and bark beetles.

• MeSH
• biodiverzita * MeSH
• borovicovité klasifikace   mikrobiologie   parazitologie   MeSH
• brouci klasifikace   mikrobiologie   MeSH
• fylogeneze MeSH
• hmyz - vektory mikrobiologie   MeSH
• hostitelská specificita MeSH
• Hypocreales klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• molekulární sekvence - údaje MeSH
• nemoci rostlin mikrobiologie   parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH