In the present study, potentiality of endophytic microorganisms such as Rigidiporus vinctus AAU EF, Trichoderma reesei UH EF, and Sphingobacterium tabacisoli UH EB in the management of panama wilt and growth promotion of banana was assessed through artificial inoculation. During the study, a total of 220 bacterial and 110 fungal endophytes were isolated from root, pseudostem, and leaf samples of banana, and they were evaluated against Fusarium oxysporum f. sp cubense causing panama wilt. Out of total 330 bacterial and fungal endophytes, only five endophytes exhibited antagonism against Fusarium oxysporum f. sp cubense, out of which only three isolates, namely Trichoderma reesei UH EF, Rigidiporus vinctus AAU EF, and Sphingobacterium tabacisoli UH EB, produced indole acetic acid, siderophore, and hydrogen cyanide, except one bacterial strain Sphingobacterium tabacisoli UH EB which does not produce hydrogen cyanide. Furthermore, these three endophytes were identified through cultural and morphological characteristics as well as by the sequencing internal transcribed spacer (ITS) and 16S rRNA gene sequences analysis for bacteria, respectively. The response of host plant to endophyte inoculation was assessed by measuring the change in four growth parameters; plant height, pseudo stem girth (diameter), number of roots, and total number of leaves. The application of endophytes, irrespective of isolate and treatment type promoted the overall growth of the plant growth when compared with diseased plants with significant higher values recorded for all parameters assessed. The endophytes reported as growth promoters were found to have significant inhibition effect on Foc which can evidenced with lowest AUDPC values and epidemic rate at 99.09 units2 and 0.02 unit/day, respectively.
- MeSH
- banánovník * mikrobiologie MeSH
- endofyty * fyziologie MeSH
- Fusarium * fyziologie MeSH
- Hypocreales fyziologie MeSH
- mikrobiální interakce fyziologie MeSH
- nemoci rostlin * mikrobiologie prevence a kontrola MeSH
- Polyporales fyziologie MeSH
- RNA ribozomální 16S genetika MeSH
- Sphingobacterium fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
This study examined the expression and role of vitellogenin (Vg) in the body of the firebug Pyrrhocoris apterus (Heteroptera, Insecta) during infection elicited by two entomopathogenic organisms, the nematode Steinernema carpocapsae and the fungus Isaria fumosorosea Infection by S. carpocapsae significantly upregulated Vg mRNA expression in the male body. The corresponding increase in Vg protein expression was also confirmed by electrophoretic and immunoblotting analyses. Remarkably, in females, the opposite tendency was noted. Nematodal infection significantly reduced both Vg mRNA and Vg protein expression levels in fat body and hemolymph, respectively. We speculate that infection of reproductive females reduces Vg expression to a level that is still sufficient for defense, but is insufficient for reproduction. This circumstance reduces energy expenditure and helps the individual to cope with the infection. Importantly, purified Vg significantly inhibited growth of Xenorhabdus spp., an entomotoxic bacteria isolated from S. carpocapsae. However, the effect of Vg against I. fumosorosea was not so obvious. The fungus significantly stimulated Vg gene expression in males; however, a similar increase was not recapitulated at the protein level. Nevertheless, in females, both mRNA and protein Vg levels were significantly reduced after the fungal infection. The obtained data demonstrate that Vg is probably an important defense protein, possibly with a specific activity. This considerably expands the known spectrum of Vg functions, as its primary role was thought to be limited to regulating egg development in the female body.
- MeSH
- exprese genu MeSH
- Heteroptera genetika metabolismus mikrobiologie parazitologie MeSH
- hmyzí proteiny genetika metabolismus MeSH
- Hypocreales fyziologie MeSH
- interakce hostitele a parazita MeSH
- interakce hostitele a patogenu genetika fyziologie MeSH
- Rhabditida fyziologie MeSH
- vitelogeniny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Species of Geosmithia are cosmopolitan but understudied fungi, and most are associated with phloem-feeding bark beetles on various woody hosts. We surveyed 207 bark and ambrosia beetles from 37 species in the eastern USA for associated fungi. The community is dominated by species in the G. pallida species complex (GPSC) and included several Geosmithia isolates that appear to be new to science. The new Geosmithia isolates exhibited the characteristic brownish-colored colonies typical for the G. pallida species complex and were phylogenetically resolved as two genealogically exclusive lineages based on a concatenated multilocus data set based on the internal transcribed spacers (ITS) of the nuc rDNA (ITS1-5.8S-ITS2 = ITS), and the translation elongation factor 1-α (TEF1-α), β-tubulin (TUB2), and RNA polymerase II second largest subunit (RPB2) genes. Two new Geosmithia species, G. brunnea and G. proliferans, are proposed, and their morphological traits and phylogenetic placements are presented.
- MeSH
- biologické pigmenty metabolismus MeSH
- DNA fungální chemie genetika MeSH
- elongační faktor 1 genetika MeSH
- fylogeneze MeSH
- Hypocreales klasifikace genetika izolace a purifikace fyziologie MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- nosatcovití mikrobiologie MeSH
- RNA ribozomální 5.8S genetika MeSH
- RNA-polymerasa II genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- tubulin genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Spojené státy americké MeSH
Fungi from the genus Geosmithia (Ascomycota: Hypocreales) are associated with bark beetles (Coleoptera: Scolytinae), though little is known about ecology, diversity, and distribution of these fungi across beetle and its host tree species. This study surveyed the diversity, distribution and vector affinity of Geosmithia isolated from subcortical insects that colonized trees from the family Pinaceae in Central and Northeastern Europe. Twelve Geosmithia species were isolated from 85 plant samples associated with 23 subcortical insect species (including 14 bark beetle species). Geosmithia community composition was similar across different localities and vector species; although the fungal communities associated with insects that colonized Pinus differed from that colonizing other tree species (Abies, Larix, and Picea). Ten Geosmithia species from four independent phylogenetic lineages were not reported previously from vectors feeding on other plant families and seem to be restricted to the vectors from Pinaceae only. We conclude that presence of such substrate specificity suggests a long and stable association between Geosmithia and bark beetles.
- MeSH
- biodiverzita * MeSH
- borovicovité klasifikace mikrobiologie parazitologie MeSH
- brouci klasifikace mikrobiologie MeSH
- fylogeneze MeSH
- hmyz - vektory mikrobiologie MeSH
- hostitelská specificita MeSH
- Hypocreales klasifikace genetika izolace a purifikace fyziologie MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin mikrobiologie parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
Widespread morbidity and mortality of Juglans nigra has occurred in the western USA over the past decade. Tree mortality is the result of aggressive feeding by the walnut twig beetle (Pityophthorus juglandis) and subsequent canker development around beetle galleries caused by a filamentous ascomycete in genus Geosmithia (Ascomycota: Hypocreales). Thirty-seven Geosmithia strains collected from J. californica, J. hindsii, J. major and J. nigra in seven USA states (AZ, CA, CO, ID, OR, UT, WA) were compared with morphological and molecular methods (ITS rDNA sequences). Strains had common characteristics including yellowish conidia en masse, growth at 37 C and absence of growth on Czapek-Dox agar and belonged to a single species described here as G. morbida. Whereas Geosmithia are common saprobes associated with bark beetles attacking hardwoods and conifers worldwide, G. morbida is the first species documented as a plant pathogen.
- MeSH
- brouci mikrobiologie fyziologie MeSH
- DNA fungální genetika MeSH
- fylogeneze MeSH
- Hypocreales klasifikace genetika izolace a purifikace fyziologie MeSH
- Juglans mikrobiologie parazitologie MeSH
- mezerníky ribozomální DNA genetika MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin mikrobiologie MeSH
- symbióza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Spojené státy americké MeSH
