Respiratory infections are a real threat for humans, and therefore the pig model is of interest for studies. As one of a case for studies, Actinobacillus pleuropneumoniae (APP) caused infections and still worries many pig breeders around the world. To better understand the influence of pathogenic effect of APP on a respiratory system-lungs and tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally infected by APP and two were used as non-infected control, and 48 cryosections have been obtained. MALDI-TOF MSI and immunohistochemistry (IHC) were used to study spatial distribution of infectious markers, especially interleukins, in cryosections of porcine tissues of lungs (necrotic area, marginal zone) and tracheobronchial lymph nodes (TBLN) from pigs infected by APP. CD163, interleukin 1β (IL‑1β) and a protegrin-4 precursor were successfully detected based on their tryptic fragments. CD163 and IL‑1β were confirmed also by IHC. The protegrin-4 precursor was identified by MALDI-TOF/TOF directly on the tissue cryosections. CD163, IL‑1β and protegrin‑4 precursor were all significantly (p < 0.001) more expressed in necrotic areas of lungs infected by APP than in marginal zone, TBLN and in control lungs.

• MeSH
• Actinobacillus pleuropneumoniae patogenita   MeSH
• antigeny diferenciační myelomonocytární metabolismus   MeSH
• biologické markery metabolismus   MeSH
• bronchy metabolismus   MeSH
• CD antigeny metabolismus   MeSH
• infekce bakteriemi rodu Actinobacillus metabolismus   mikrobiologie   MeSH
• infekce dýchací soustavy metabolismus   mikrobiologie   MeSH
• interleukin-1beta metabolismus   MeSH
• kationické antimikrobiální peptidy metabolismus   MeSH
• lymfatické uzliny metabolismus   MeSH
• plíce metabolismus   MeSH
• prasata MeSH
• receptory buněčného povrchu metabolismus   MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH