The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHCα was represented by the lower band with higher mobility and MyHCβ by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHCα possessed a higher mobility rate than the MyHCβ isoform. Our data highlighted the importance of the careful identification of the MyHCα and MyHCβ isoforms analyzed by the SDS-PAGE.

• MeSH
• elektroforéza v polyakrylamidovém gelu metody   MeSH
• hypertyreóza metabolismus   MeSH
• hypotyreóza metabolismus   MeSH
• komorové myosiny chemie   izolace a purifikace   metabolismus   MeSH
• krysa rodu rattus MeSH
• molekulární sekvence - údaje MeSH
• potkani inbrední LEW MeSH
• protein - isoformy MeSH
• sekvence aminokyselin MeSH
• sekvenční seřazení MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• těžké řetězce myosinu chemie   izolace a purifikace   metabolismus   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH