11β-Hydroxysteroid dehydrogenase type 1 (11HSD1) is a microsomal NADPH-dependent oxidoreductase which elevates intracellular concentrations of active glucocorticoids. Data obtained from mouse strains with genetically manipulated 11HSD1 showed that local metabolism of glucocorticoids plays an important role in the development of metabolic syndrome. Tissue specific dysregulation of 11HSD1 was also found in other models of metabolic syndrome as well as in a number of clinical studies. Here, we studied local glucocorticoid action in the liver, subcutaneous adipose tissue (SAT) and skeletal muscles of male and female Prague hereditary hypertriglyceridemic rats (HHTg) and their normotriglyceridemic counterpart, the Wistar rats. 11HSD1 bioactivity was measured as a conversion of [(3)H]11-dehydrocorticosterone to [(3)H]corticosterone or vice versa. Additionally to express level of active 11HSD1 protein, enzyme activity was measured in tissue homogenates. mRNA abundance of 11HSD1, hexoso-6-phosphate dehydrogenase (H6PDH) and the glucocorticoid receptor (GR) was measured by real-time PCR. In comparison with normotriglyceridemic animals, female HHTg rats showed enhanced regeneration of glucocorticoids in the liver and the absence of any changes in SAT and skeletal muscle. In contrast to females, the glucocorticoid regeneration in males of HHTg rats was unchanged in liver, but stimulated in SAT and downregulated in muscle. Furthermore, SAT and skeletal muscle exhibited not only 11-reductase but also 11-oxidase catalyzed by 11HSD1. In females of both strains, 11-oxidase activity largely exceeded 11-reductase activity. No dramatic changes were found in the mRNA expression of H6PDH and GR. Our data provide evidence that the relationship between hypertriglyceridemia and glucocorticoid action is complex and gender specific.
- MeSH
- 11-beta-hydroxysteroiddehydrogenasa typ 1 metabolismus MeSH
- glukokortikoidy metabolismus MeSH
- hypertriglyceridemie enzymologie metabolismus MeSH
- játra enzymologie metabolismus MeSH
- karbohydrátdehydrogenasy metabolismus MeSH
- kortikosteron analogy a deriváty metabolismus MeSH
- kosterní svaly enzymologie metabolismus MeSH
- krysa rodu rattus MeSH
- metabolický syndrom enzymologie MeSH
- modely nemocí na zvířatech MeSH
- podkožní tuk enzymologie metabolismus MeSH
- potkani Wistar MeSH
- receptory glukokortikoidů genetika metabolismus MeSH
- sexuální faktory MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The objective of the study was to investigate the effects of sex and slaughter age on the fatty acid (FA) composition and stearoyl-CoA desaturase gene expression in muscle and adipose tissue. Twenty-four Charolais × Simmental crossbred bulls and heifers were raised under similar conditions and slaughtered at 14 or 18 months of age. The total amount of FA in muscle increased markedly in older animals with higher contents of monounsaturated FA (MUFA) in heifers than bulls. The proportions of MUFA and desaturation indices were higher in heifers, whereas polyunsaturated FA were higher in bulls in both muscle and subcutaneous adipose tissue. The results of this study demonstrated sex-dependent differences in the FA composition of muscle and subcutaneous adipose tissue from cattle slaughtered at different ages. The expression of the stearoyl-CoA desaturase gene was higher in the adipose tissue of heifers compared to bulls, and its variation partly contributed to sex- and age-differences in the FA composition of bovine adipose tissue.
- MeSH
- kosterní svaly chemie enzymologie MeSH
- kyseliny mastné mononenasycené analýza MeSH
- mastné kyseliny analýza MeSH
- messenger RNA genetika metabolismus MeSH
- nenasycené mastné kyseliny analýza MeSH
- podkožní tuk chemie enzymologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- regulace genové exprese MeSH
- sexuální faktory MeSH
- skot MeSH
- stearyl-CoA-desaturasa genetika metabolismus MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
