Only very few comparative studies have been performed that evaluate general trends of virus growth under 3D in comparison with 2D cell culture conditions. The aim of this study was to investigate differences when four animal viruses are cultured in 2D and 3D. Suid herpesvirus 1 (SuHV-1), Vesicular stomatitis virus (VSIV), Bovine adenovirus (BAdV) and Bovine parainfluenza 3 virus (BPIV-3) were cultivated in 3D rotating wall vessels (RWVs) and conventional 2D cultures. The production of virus particles, the portion of infectious particles, and the infectious growth curves were compared. For all viruses, the production of virus particles (related to cell density), including the non-infectious ones, was lower in 3D than in 2D culture. The production of only infectious particles was significantly lower in BAdV and BPIV-3 in 3D cultures in relation to cell density. The two cultivation approaches resulted in significantly different virus particle-to-TCID50 ratios in three of the four viruses: lower in SuHV-1 and BPIV-3 and higher in BAdV in 3D culture. The infectious virus growth rates were not significantly different in all viruses. Although 3D RWV culture resulted in lower production of virus particles compared to 2D systems, the portion of infectious particles was higher for some viruses.
- MeSH
- Atadenovirus růst a vývoj fyziologie ultrastruktura MeSH
- buněčné kultury * přístrojové vybavení metody MeSH
- buňky MDCK MeSH
- Cercopithecus aethiops MeSH
- kultivace virů metody MeSH
- prasata MeSH
- prasečí herpesvirus 1 růst a vývoj fyziologie ultrastruktura MeSH
- psi MeSH
- replikace viru MeSH
- skot MeSH
- Vero buňky MeSH
- virus bovinní parainfluenzy 3 růst a vývoj fyziologie ultrastruktura MeSH
- virus vezikulární stomatitidy, kmen Indiana růst a vývoj fyziologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Hollow-core particles, forming crystals in nuclei, prevailed in HeLa cells infected with an attenuated strain of pseudorabies virus (PRV). After infection with a virulent PRV strain, the cells contained mainly fully infectious dense-core particles. These findings might explain the lower susceptibility of HeLa and some other human cells to infection with attenuated strains of PRV as compared to virulent strains.
- MeSH
- buněčné inkluze virové MeSH
- buněčné jádro mikrobiologie MeSH
- cytopatogenní efekt virový MeSH
- cytoplazma mikrobiologie MeSH
- elektronová mikroskopie MeSH
- HeLa buňky MeSH
- Herpesviridae růst a vývoj MeSH
- krystalografie MeSH
- prasečí herpesvirus 1 patogenita růst a vývoj ultrastruktura MeSH
- replikace viru MeSH
- virulence MeSH
- MeSH
- buněčné inkluze virové MeSH
- buněčné jádro mikrobiologie MeSH
- cytopatogenní efekt virový MeSH
- cytoplazma mikrobiologie MeSH
- elektronová mikroskopie MeSH
- HeLa buňky MeSH
- Herpesviridae růst a vývoj MeSH
- krystalografie MeSH
- prasečí herpesvirus 1 patogenita růst a vývoj ultrastruktura MeSH
- replikace viru MeSH
- virulence MeSH
