We investigated the effect of pertussis toxin (PTX) on hypotensive response induced by acetylcholine (ACh) and bradykinin (BK) and on noradrenaline (NA)-induced pressor response in spontaneously hypertensive rats (SHR). Fifteen-week-old Wistar rats and age-matched SHR were used. Half of SHR received PTX (10 microg/kg/i.v.) and the experiments were performed 48 h later. After the anesthesia the right carotid artery was cannulated in order to record blood pressure (BP). The hypotensive response to ACh was enhanced in SHR compared to Wistar rats. After pretreatment of SHR with PTX the hypotensive response to ACh was reduced compared to untreated SHR and it was also diminished in comparison to Wistar rats. Similarly, the hypotensive response to BK was also decreased after PTX pretreatment. The pressor response to NA was increased in SHR compared to Wistar rats. NA-induced pressor response was considerably decreased after PTX pretreatment compared to untreated SHR. In conclusion, the enhancement of hypotensive and pressor responses in SHR was abolished after PTX pretreatment. Our results suggested that the activation of PTX-sensitive inhibitory G(i) proteins is involved in the regulation of integrated vasoactive responses in SHR and PTX pretreatment could be effectively used for modification of BP regulation in this type of experimental hypertension.

• MeSH
• acetylcholin MeSH
• analýza párové shody MeSH
• bradykinin MeSH
• fyziologická adaptace účinky léků   MeSH
• hemodynamika fyziologie   účinky léků   MeSH
• hypotenze chemicky indukované   patofyziologie   MeSH
• inhibitory enzymů MeSH
• krevní tlak genetika   účinky záření   MeSH
• krysa rodu rattus MeSH
• noradrenalin metabolismus   MeSH
• pertusový toxin farmakologie   MeSH
• potkani inbrední SHR MeSH
• potkani Wistar MeSH
• proteiny vázající GTP - alfa-podjednotky Gi-Go metabolismus   účinky léků   MeSH
• signální transdukce fyziologie   účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH