Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe(2)O(3)) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe(2)O(3) surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe(2)O(3) and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe(2)O(3) nanoparticles with DPA-HA/γ-Fe(2)O(3) = 0.45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.

• MeSH
• biokompatibilní potahované materiály chemie   MeSH
• buněčná diferenciace MeSH
• chondrocyty cytologie   MeSH
• dopamin chemie   MeSH
• endocytóza MeSH
• ferrokyanidy MeSH
• kontrastní látky diagnostické užití   MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• kyselina hyaluronová chemie   MeSH
• lidé MeSH
• magnetická rezonanční tomografie MeSH
• magnetické nanočástice chemie   diagnostické užití   ultrastruktura   MeSH
• mezenchymální kmenové buňky cytologie   účinky léků   MeSH
• nanomedicína MeSH
• transmisní elektronová mikroskopie MeSH
• velikost částic MeSH
• viabilita buněk MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH