Phytophthora is arguably one of the most damaging genera of plant pathogens. This pathogen is well suited to transmission via the international plant trade, and globalization has been promoting its spread since the 19th century. Early detection is essential for reducing its economic and ecological impact. Here, a shotgun proteomics approach was utilized for Phytophthora analysis. The collection of 37 Phytophthora isolates representing 12 different species was screened for species-specific peptide patterns. Next, Phytophthora proteins were detected in planta, employing model plants Solanum tuberosum and Hordeum vulgare. Although the evolutionarily conserved sequences represented more than 10% of the host proteome and limited the pathogen detection, the comparison between qPCR and protein data highlighted more than 300 protein markers, which correlated positively with the amount of P. infestans DNA. Finally, the analysis of P. palmivora response in barley revealed significant alterations in plant metabolism. These changes included enzymes of cell wall metabolism, ROS production, and proteins involved in trafficking. The observed root-specific attenuation in stress-response mechanisms, including the biosynthesis of jasmonates, ethylene and polyamines, and an accumulation of serotonin, provided the first insight into molecular mechanisms behind this particular biotic interaction.

• MeSH
• chromatografie kapalinová MeSH
• fyziologický stres MeSH
• hmotnostní spektrometrie MeSH
• ječmen (rod) enzymologie   metabolismus   mikrobiologie   MeSH
• listy rostlin metabolismus   mikrobiologie   MeSH
• metabolické sítě a dráhy MeSH
• nemoci rostlin mikrobiologie   MeSH
• peptidy metabolismus   MeSH
• Phytophthora infestans genetika   izolace a purifikace   MeSH
• polymerázová řetězová reakce MeSH
• proteom metabolismus   MeSH
• proteomika MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• rostlinné proteiny metabolismus   MeSH
• Solanum tuberosum metabolismus   mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH

Hydrogen peroxide promotes seed germination, but the molecular mechanisms underlying this process are unclear. This study presents the results of eggplant (Solanum melongena) germination analyses conducted at two different temperatures and follows the effect of hydrogen peroxide treatment on seed germination and the seed proteome. Hydrogen peroxide was found to promote eggplant germination in a way not dissimilar to that of increased temperature stimuli. LC-MS profiling detected 729 protein families, 77 of which responded to a temperature increase or hydrogen peroxide treatment. These differentially abundant proteins were found to be involved in a number of processes, including protein and amino acid metabolism, carbohydrate metabolism, and the glyoxylate cycle. There was a very low overlap between hydrogen peroxide and temperature-responsive proteins, highlighting the differences behind the seemingly similar outcomes. Furthermore, the observed changes from the seed proteome indicate that hydrogen peroxide treatment diminished the seed endogenous hydrogen peroxide pool and that a part of manifested positive hydrogen peroxide effect might be related to altered sensitivity to abscisic acid.

• MeSH
• chromatografie kapalinová MeSH
• fyziologický stres účinky léků   MeSH
• hmotnostní spektrometrie MeSH
• klíčení účinky léků   MeSH
• metabolismus sacharidů účinky léků   MeSH
• peroxid vodíku farmakologie   MeSH
• regulace genové exprese u rostlin účinky léků   MeSH
• rostlinné proteiny metabolismus   MeSH
• Solanum melongena účinky léků   fyziologie   MeSH
• teplota MeSH
• vývojová regulace genové exprese účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH