CRISPR is a prokaryotic defence system that was adapted as a tool for genome editing and has become one of the most important discoveries of this century. CRISPR-associated endonucleases cleave DNA at precise sites, which are marked by complementary short-guided RNA. The recently developed versions of endonucleases are compatible with a broad range of PAM motifs, have a higher specificity and enable a specific nucleotide to be replaced.

• MeSH
• DNA genetics   MeSH
• Gene Editing * MeSH
• Endonucleases genetics   metabolism   MeSH
• RNA, Guide, Kinetoplastida genetics   MeSH
• Humans MeSH
• Clustered Regularly Interspaced Short Palindromic Repeats genetics   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH