The stringent response enables bacteria to respond to nutrient limitation and other stress conditions through production of the nucleotide-based second messengers ppGpp and pppGpp, collectively known as (p)ppGpp. Here, we report that (p)ppGpp inhibits the signal recognition particle (SRP)-dependent protein targeting pathway, which is essential for membrane protein biogenesis and protein secretion. More specifically, (p)ppGpp binds to the SRP GTPases Ffh and FtsY, and inhibits the formation of the SRP receptor-targeting complex, which is central for the coordinated binding of the translating ribosome to the SecYEG translocon. Cryo-EM analysis of SRP bound to translating ribosomes suggests that (p)ppGpp may induce a distinct conformational stabilization of the NG domain of Ffh and FtsY in Bacillus subtilis but not in E. coli.

• MeSH
• bakteriální proteiny metabolismus   MeSH
• Escherichia coli metabolismus   MeSH
• guanosinpentafosfát metabolismus   MeSH
• proteiny z Escherichia coli * metabolismus   MeSH
• receptory cytoplazmatické a nukleární metabolismus   MeSH
• signál-rozpoznávající částice * metabolismus   MeSH
• vazba proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

PURPOSE OF THE STUDY Clinical results of long-term follow-up after traumatic periprosthetic femur fractures and different therapies (ORIF vs. revision arthroplasty) MATERIAL AND METHODS The Visual Analog Scale (VAS), Harris-Hip-Score (HHS), Oxford-Hip-Score (OHS), Oxford-Knee-Score (OKS), Knee-Society-Score (KSS), SF-36 Questionnaire and Funktionsfragebogen Hannover (FFH) were used to evaluate outcome and functionality. Radiological examinations were performed and the Vancouver (THA) and Lewis and Rorabeck (TKA) classifications used. RESULTS 70 patients suffered a periprosthetic hip fracture (29× revision prosthesis, 41x ORIF), 23 patients underwent an ORIF due to periprosthetic fracture of a TKA (total mean age 75.2 years). 47 patients (follow-up rate 51%) were examined 40 months after surgery (mean age 72 years) (THA: 16× revision, 23× ORIF, TKA: 8× ORIF). The VAS revealed significant less pain in the group that had undergone revision hip arthroplasty than in the ORIF group: 3.9±1 vs. 5.1±1.7 (p<0.05), respectively. 5/16 patients with revision arthroplasty had excellent or good results in the HSS compared to 3/23 patients after ORIF. The OHS yielded excellent or good results in 12/16 patients after revision arthroplasty vs. 10/23 after ORIF. The VAS after ORIF in patients who suffered periprosthetic knee fractures was 4.9±2.1. 3/8 patients achieved excellent or good results according to the OKS. CONCLUSION Every functional score (HSS, OHS, FFH, SF-36) of those patients who had undergone revision arthroplasty was slightly higher and their VAS significantly lower than the scores of the patients after ORIF. Key words: periprosthetic fractures, trauma, open reduction and internal fixation, revision arthroplasty.

• MeSH
• lidé MeSH
• náhrada kyčelního kloubu MeSH
• otevřená repozice fraktury MeSH
• periprotetické fraktury diagnostické zobrazování   patofyziologie   chirurgie   MeSH
• poranění kyčle patofyziologie   chirurgie   MeSH
• reoperace MeSH
• senioři MeSH
• skóre závažnosti úrazu MeSH
• vnitřní fixace fraktury MeSH
• výsledek terapie MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

• MeSH
• chemie MeSH

• Konspekt
• Organická chemie
• NLK Obory
• chemie, klinická chemie
• NLK Publikační typ
• učebnice vysokých škol