Propofol has been shown to against intestinal reperfusion injury when treated either before or after ischemia, during which mast cell could be activated. The aim of this study was to evaluate the role of propofol in restoring the intestinal epithelial cells integrity disrupted by mast cell activation or the released tryptase after activation in vitro. We investigated the effect of: (1) tryptase on Caco-2 monolayers in the presence of PAR-2 inhibitor or propofol, (2) mast cell degranulation in a Caco-2/LAD-2 co-culture model in the presence of propofol, and (3) propofol on mast cell degranulation. Epithelial integrity was detected using transepithelial resistance (TER) and permeability to fluorescein isothiocyanate (FITC)-dextran (the apparent permeability coefficient, Papp). The expression of junctional proteins zonula occludens-1 (ZO-1/TJP1) and occludin were determined using western blot analysis and immunofluorescence microscopy. The intracellular levels of reactive oxidative species (ROS) and Ca2+ were measured using flow cytometry. Tryptase directly enhanced intestinal barrier permeability as demonstrated by significant reductions in TER, ZO-1, and occludin protein expression and concomitant increases in Papp. The intestinal barrier integrity was restored by PAR-2 inhibitor but not by propofol. Meanwhile, mast cell degranulation resulted in epithelial integrity disruption in the Caco-2/LAD-2 co-culture model, which was dramatically attenuated by propofol. Mast cell degranulation caused significant increases in intracellular ROS and Ca(2+) levels, which were blocked by propofol and NAC. Propofol pretreatment can inhibit mast cell activation via ROS/Ca(2+) and restore the intestinal barrier integrity induced by mast cell activation, instead of by tryptase.

• MeSH
• Caco-2 buňky MeSH
• degranulace buněk MeSH
• epitelové buňky metabolismus   MeSH
• lidé MeSH
• mastocyty metabolismus   MeSH
• okludin metabolismus   MeSH
• permeabilita MeSH
• propofol * farmakologie   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• střevní sliznice metabolismus   MeSH
• tryptasy metabolismus   farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• analýza rozptylu MeSH
• dospělí MeSH
• Gravesova nemoc farmakoterapie   krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• methimazol aplikace a dávkování   terapeutické užití   MeSH
• mladiství MeSH
• monoklonální protilátky krev   MeSH
• propylthiouracil aplikace a dávkování   terapeutické užití   MeSH
• receptory thyreotropinu imunologie   MeSH
• rozvrh dávkování léků MeSH
• thyreostatika aplikace a dávkování   terapeutické užití   MeSH
• thyroxin krev   MeSH
• trijodthyronin krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• souhrny MeSH