Multi-virus-specific T cell lines
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Adoptive transfer of multivirus-specific T cell lines (MVST) is an advanced tool for immunotherapy of virus infections after hematopoietic stem cell transplantation (HSCT). Their preparation includes activation of donor virus-specific T cells by the mixture of oligopeptides derived from immunodominant antigens of several most harmful viruses, i.e. human cytomegalovirus (HCMV), polyomavirus BK (BKV), Epstein-Barr virus (EBV) and adenovirus (ADV). The aim of our study was to find out whether antigenic competition may have an impact on the expansion of virus-specific T cells. MVST from several heathy blood donors were generated using a pulse of overlapping oligopeptides (PepMixes™, derived from the IE1 and pp65 CMV antigens, VP1 and LTAG BKV antigens, BZLF1 and EBNA1 proteins of EBV and hexon protein from ADV) and short time culture in the presence of IL-7 and IL-4. The amount of virus-specific T cells in MVST was measured by ELISPOT and flow cytometry after re-stimulation with individual antigens. To evaluate antigenic competition, MVST were expanded either with a complete set of antigens or with the mixture lacking some of them. MVST expanded with the antigen mixture including CMV antigens contained a lower proportion of the T cells of other antigen specificities. A similar inhibitory effect was not apparent for EBV-derived peptides. The competitive effect of CMV antigens was most pronounced in MVST from CMV-seropositive donors and was mediated by both IE1 and pp65-derived peptides. Antigenic competition did not influence the phenotype of either CMV- or BKV-specific T cells. Both T cell populations had an effector memory phenotype (CD45RO+, CD27-, CCR7-). However, CMV-specific T cells preferentially consist of CD8+ while in BKV-specific T cells, the CD4+ phenotype predominated. Modification of the MVST manufacture protocol to prevent antigenic competition may increase the efficacy of MVST in therapy of BKV infections in HSCT recipients.
- MeSH
- Adenoviridae imunologie patogenita MeSH
- adenovirové infekce lidí imunologie terapie virologie MeSH
- aktivace lymfocytů MeSH
- antigeny virové imunologie MeSH
- cytomegalovirové infekce imunologie terapie virologie MeSH
- Cytomegalovirus imunologie patogenita MeSH
- fenotyp MeSH
- imunodominantní epitopy MeSH
- imunoterapie adoptivní * MeSH
- infekce onkogenními viry imunologie terapie virologie MeSH
- infekce virem Epsteina-Barrové imunologie terapie virologie MeSH
- interakce hostitele a patogenu MeSH
- kultivované buňky MeSH
- lidé MeSH
- polyomavirové infekce imunologie terapie virologie MeSH
- T-lymfocyty imunologie transplantace virologie MeSH
- transplantace hematopoetických kmenových buněk škodlivé účinky MeSH
- virové nemoci imunologie terapie virologie MeSH
- virus BK imunologie patogenita MeSH
- virus Epsteinův-Barrové imunologie patogenita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Eternal life : cell immortalization and tumorigenesis -- 11. Multi-step tumorigenesis -- 12. ... ... 72 -- Rous sarcoma virus is discovered to transform infected cells in culture 75 -- The continued presence ... ... -- 8.2 Cells make decisions about growth and quiescence during a specific period in the Gi phase 281 ... ... types 579 -- 13.2 The cells forming cancer cell lines develop without heterotypic interactions and deviate ... ... of killing used by the immune system 773 -- 15.17 Cancer cells attract regulator)\' T cells to fend ...
2nd ed. 1 sv. (různé stránkování) : il. ; 28 cm + 1 plakát, 1 DVD-ROM
- MeSH
- biologie buňky MeSH
- genetická predispozice k nemoci MeSH
- molekulární biologie MeSH
- nádorové procesy MeSH
- nádory MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Publikační typ
- monografie
... T. ... ... Devilee % 296 -- A European cancer cell line resource, A. ... ... Schwartz 406 xiv -- Cell-cell, cell-matrix interactions in atherosclerotic plaque and smooth muscle differentiation ... ... Will 476 -- Concerted action on T-cell autoimmunity in multiple sclerosis, L. ... ... Vrensen 594 -- The human haematopoietic stem cell, S. ...
Biomedical and health research, ISSN 0929-6743 vol. 9
xxxix, 744 s. ; 24 cm
