Multicomponent analysis
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Práca bola zameraná na vypracovanie a optimalizáciu pracovných podmienok pri identifikácii a stanovení zinku a medi v polykomponentných minerálnych prípravkoch. Zinok Walmark® a TrikrátSedem FeCuZn modernou elektrochemickou analytickou metódou – galvanostatickou rozpúšťacou chronopotenciometriou (SCP) a nukleárnou analytickou metódou – rádionuklidovou röntgenofluorescenčnou analýzou (RRFA). Správnosť výsledkov analýz bola potvrdená porovnaním stanovení obsahov prvkov s použitím oboch analytických metód.
The aim of this study was to elaborate and optimize the working conditions for the identification and determination of zinc and copper present in the multicomponent mineral preparations Zinok Walmark® and TrikrátSedem FeCuZn by a modern electrochemical analytical method – galvanostatic stripping chronopotentiometry, and a nuclear analytical method – X-ray fluorescence analysis. The proposed analytical methods were compared with each other and the obtained results concerning the quantity of determined elements were in good agreement.
Úvod. Multikomponentní odběry jsou moderní alternativou odběrů dárců krve, jednotná výběrová kriteria pro dárce dosud nebyla stanovena. Cílem práce bylo tato kritéria blíže upřesnit. Metoda. U provedených multikomponentních odběrů bylo provedeno vzájemné posouzení vstupních parametrů dárců krve, parametrů aferéz a jakosti získaných transfuzních přípravků, byl zhodnocen význam výběrových kritérií pro jednotlivé typy multikomponentních odběrů. Výsledky. U 137 dárců bylo provedeno 250 odběrů na separátoru Haemonetics MCS+ a 44 odběrů na separátoru TRIMA Accel (dvojité erytrocytaferézy a kombinované odběry trombocytů). Odběry trvaly 36–81 minut podle typu protokolu, odebíraný objem byl 7–13 % celkového objemu krve (TBV) u obou separátorů, jakost přípravků splnila požadovaná kritéria. U trombocytaferéz s plazmou byl průměrný předodběrový počet trombocytů dárce významně nižší u separátoru TRIMA Accel, TBV dárce neměl vliv na trvání aferézy ani na jakost přípravků. Diskuse. Výsledky práce ukazují na nutnost vybírat dárce diferencovaně podle jenotlivých přístrojů a protokolů, klíčovým parametrem pro výběr dárce pro kombinované trombocytaferézy je předodběrový počet trombocytů dárce, hodnota TBV je limitujícím faktorem zejména pro volbu objemu odebíraných přípravků. Použití výběrových kritérií Rady Evropy pro dvojité erytrocytaferézy je třeba doplnit o monitorování zásob železa dárce krve.
Introduction. Multicomponent collection belongs to the modern alternative of the blood donation. Uniform criteria for selection of donors have not been established yet. The aim of this study was to specify these criteria. Methods. The reciprocal analysis of entry parameters, apheretic parameters, and quality of blood components were compared in performed multicomponent collections. Significance of selected criteria for individual types of multicomponent collections was evaluated. Results. Two hundred fifty collections were performed in 137 blood donors using Haemonetics MCS+ separator and 44 collections using TRIMA Accel separator (2 unit red cell apheresis and combined thrombocytapheresis). The collections lasted from 31 to 81 minutes according to the type of protocol. The collected volume was 7 to 13 % from the total blood volume (TBV) in both separators, and the quality of components met required criteria. Using TRIMA Accel, the mean platelet count in blood donors before donation was significantly lower, and TBV did not affect duration of apheresis nor quality of the components. Discussion. Results of this study indicate the necessity of the selection of blood donors in accordance to both devices and protocols. Key factor for the selection of blood donors for combined thrombocytapheresis is platelet count before donation. The value of TBV is one of the limiting factors particularly for selection of the volume of the components. Selection criteria of Council of Europe for 2 unit red blood cell apheresis are necessary to be completed about monitoring of blood donor storage iron.
Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.
- MeSH
- chromozomy hub fyziologie MeSH
- mapy interakcí proteinů fyziologie MeSH
- multiproteinové komplexy metabolismus MeSH
- podjednotky proteinů metabolismus MeSH
- proteiny buněčného cyklu metabolismus MeSH
- Saccharomyces cerevisiae - proteiny metabolismus MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- techniky dvojhybridového systému MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Exercise-based cardiac rehabilitation is a highly recommended intervention towards the advancement of the cardiovascular disease (CVD) patients' health profile; though with low participation rates. Although home-based cardiac rehabilitation (HBCR) with the use of wearable sensors is proposed as a feasible alternative rehabilitation model, further investigation is needed. This systematic review and meta-analysis aimed to evaluate the effectiveness of wearable sensors-assisted HBCR in improving the CVD patients' cardiorespiratory fitness (CRF) and health profile. PubMed, Scopus, Cinahl, Cochrane Library, and PsycINFO were searched from 2010 to January 2022, using relevant keywords. A total of 14 randomized controlled trials, written in English, comparing wearable sensors-assisted HBCR to center-based cardiac rehabilitation (CBCR) or usual care (UC), were included. Wearable sensors-assisted HBCR significantly improved CRF when compared to CBCR (Hedges' g = 0.22, 95% CI 0.06, 0.39; I2 = 0%; p = 0.01), whilst comparison of HBCR to UC revealed a nonsignificant effect (Hedges' g = 0.87, 95% CI -0.87, 1.85; I2 = 96.41%; p = 0.08). Effects on physical activity, quality of life, depression levels, modification of cardiovascular risk factors/laboratory parameters, and adherence were synthesized narratively. No significant differences were noted. Technology tools are growing fast in the cardiac rehabilitation era and promote exercise-based interventions into a more home-based setting. Wearable-assisted HBCR presents the potential to act as an adjunct or an alternative to CBCR.
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Phytohormones are key regulators in various physiological processes of plant growth and development. Their chemical analyses, together with their genomics and proteomics, are an integral part of plant development studies. The information on hormone levels is often valuable for biologists dealing with any hormone-regulated processes. Plant tissue is a complex multicomponent mixture containing phytohormones in minute quantities (pg or ng/g fresh weight) along with many other related compounds with similar structures and/or physicochemical properties. Therefore, their analysis requires rapid, sensitive and sufficiently selective analytical methods. The significance of solid-phase extraction for purification and hyphenated techniques such as GC-MS, LC-MS and CE-MS for routine analyses of phytohormones is discussed.
- MeSH
- Brassica MeSH
- chemické techniky analytické MeSH
- chromatografie kapalinová MeSH
- cyklopentany analýza MeSH
- cytokininy analýza MeSH
- elektroforéza kapilární MeSH
- financování organizované MeSH
- fyziologie rostlin MeSH
- gibereliny analýza MeSH
- kyselina abscisová analýza MeSH
- kyseliny indoloctové analýza MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- rostliny MeSH
- steroidy MeSH
Objective Negative effects (NEs) in group treatments remain an under-researched area. This study aimed to explore the prevalence of various types of NEs in a multicomponent group-based treatment and to determine their predictors. Method: A total of 330 patients participating in a multicomponent group-based treatment were recruited across seven clinical sites. At the end of treatment, the Negative Effects Questionnaire (NEQ) was used to measure NEs. Item-level descriptive analysis was conducted to explore the prevalence of various types of NEs, and structural equation modeling was used to determine predictors of these NEs. Results: The most frequently reported type of NEs was the worsening of symptoms, and the single most frequently reported item was the resurfacing of unpleasant memories. Predictors of NEs included the overall distress level, alexithymia, attachment avoidance, low working alliance, problem actuation, and worse outcomes; psychological mindedness was a protective factor. Conclusion: Patients who experience higher levels of distress at the beginning of treatment, who perceive the group working alliance as problematic, and who experience high in-session emotional arousal related to their problem seem to be especially prone to reporting NEs. Furthermore, the findings do not support the assumption that NEs are a prerequisite for therapeutic change.Trial registration: ISRCTN.org identifier: ISRCTN13532466.
- MeSH
- deprese * psychologie MeSH
- lidé MeSH
- prevalence MeSH
- průzkumy a dotazníky MeSH
- psychický stres * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of the present article was to perform a systematic review with meta-analysis of available scientific evidence regarding the role of different intravenous lipid emulsions (ILE) in the pathogenesis of cholestasis and parenteral nutrition-associated liver disease. A systematic review of the literature (up to March 2015) identified 23 randomized controlled trials (RCTs). Of these, 17 were performed in preterm infants or critically ill neonates with a short duration of intervention, 2 in older children with short-term use (following surgery or bone marrow transplantation), 1 in neonates with long-term use, and 3 in infants and children receiving long-term parenteral nutrition (PN). Meta-analysis showed no differences in the rate of cholestasis or bilirubin levels associated with short-term use of different ILEs. Because of high heterogeneity of the long-term studies no meta-analysis could be performed. Available studies found that the use of multicomponent fish oil (FO)-containing ILE compared with pure soya bean oil (SO), ILE-reduced liver enzymes, and bilirubin levels in noncholestatic children on long-term PN and one other RCT found that FO-based ILE-reversed cholestasis in a proportion of patients. The ESPGHAN Committee on Nutrition concludes that there is no evidence of a difference in rates of cholestasis or bilirubin levels between different ILE for short-term use in neonates. The use of multicomponent FO-containing ILE may contribute to a decrease in total bilirubin levels in children with IF on prolonged PN. Well-designed RCTs are, however, lacking and long-term effects have not been determined.
- MeSH
- cholestáza epidemiologie etiologie MeSH
- dítě MeSH
- hodnocení rizik MeSH
- jaterní testy MeSH
- kojenec MeSH
- lidé MeSH
- novorozenec MeSH
- parenterální výživa MeSH
- poradní výbory MeSH
- předškolní dítě MeSH
- randomizované kontrolované studie jako téma MeSH
- tukové emulze intravenózní aplikace a dávkování škodlivé účinky toxicita MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- metaanalýza MeSH
- přehledy MeSH
- Geografické názvy
- Evropa epidemiologie MeSH
